Harvard NeuroDiscovery Center - Optical Imaging Program
Location: Optical Imaging Facility Goldenson Building, Room 500/501 220 Longwood Ave Boston, MA 02115
| Core Summary: |
The NeuroDiscovery Optical Imaging Program provides access to advanced confocal, multi-photon and wide field microscopy. The facility specializes in various forms of fluorescence, brightfield, DIC (Differential Interference Contrast) and live cell imaging. We offer users comprehensive training (data acquisition and image analysis), assistance in the design of experimental protocols and ongoing consultation and support.
Questions regarding cell and tissue fixation techniques, fluorophore selection and immunostaining principles are always welcome, as are requests for instruction in live cell and tissue imaging. We offer flexible and customized training and support to both established and new users throughout the NeuroDiscovery community.
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| Personnel/Contact Information: |
Member: Daniel Tom
Role: Optical Imaging Research Assistant
Phone: 617-432-1442
Email
Member: Lai Ding, PhD
Role: Optical Imaging Manager
Phone: 617-432-2799
Email
| Facilities and Equipment: |
Location of Core: Optical Imaging Facility Goldenson Building, Room 500/501 220 Longwood Ave Boston, MA 02115
Major Equipment:
- 3-D for LSM
- Coherent Chameleonâ„¢ Multi-photon Excitation (MPE) laser
- Coherent Innova® Enterprise™ II ion laser system
- Hamamatsu EMCCD Camera
- Imaris Bitplane
- IP Lab
- Live cell microscopy temperature control system with perfusion
- Metamorph Imaging System
- Motorized XY stage for tiling large tissue sections
- Olympus spinning disk confocal microscope
- VWR tissue culture incubator (37°C w/ 5% CO2) for short-term storage
- Zeiss LSM 510 META upright confocal microscopes
- Zeiss LSM Software package
| Departmental Web Link: |
http://www.neurodiscovery.harvard.edu/resources/optical_2.html