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Nikon Imaging Center (HMS)

Location: Harvard Medical School, LHRRB room 113, Boston, MA

Core Summary:

The mission of the NIC@HMS is to: enhance basic research by providing access to state-of-the-art microscopy and imaging equipment, provide training courses on basic and advanced light microscopy techniques for the benefit of HMS and the greater Boston research community, introduce the latest innovations in light microscopy and imaging to the HMS community, serve as a learning center for our corporate partners and contributions, and provide a dedicated NIC@HMS director for ongoing technical consultation and support.

Institutions:
  • Harvard Medical School
Personnel/Contact Information:

Member: Waters, Jennifer, Ph.D.
Role: Director of Microscopy
Phone: (617) 432-3542
Email

Member: General Inquiries
Email

Facilities and Equipment:

Location of Core: Harvard Medical School, LHRRB room 113, Boston, MA

Major Equipment:

  • 80i (Station 6)
  • A1R confocal (Station 14)
  • C1 confocal (Station 3)
  • EZ-C1
  • MetaMorph
  • Microinjection (Station 7)
  • Nikon Ti Automated Inverted Microscope w/ Perfect Focus & 37°C Incubation Chamber (Station 12)
  • Nikon Ti Automated Inverted Microscope w/ Perfect Focus & 37°C Incubation Chamber (Station 5)
  • Nikon Ti w/ Spinning Disk Confocal, Perfect Focus & 37°C Incubation Chamber (Station 1)
  • NIS-Elements
  • PC Computer Workstations for Image Analysis
  • SDC, widefield and photobleaching (Station 2)
  • Spinning Disk Confocal (Station 10)
  • Ti (Station 8)
  • TIRF/N-STORM (Station 4)
  • TIRF with incubation enclosure (Station 15)
  • Ti with incubation enclosure (Station 9)
Services:
  • Biosensors, FRET, Ca2+ imaging and other ratiometric imaging

    This technique can be performed on the following stations:
    * Station 8 - Nikon Ti Inverted Fluorescence Microscope w/ Perfect Focus (LHRRB 113)
    * Station 9 - Nikon Ti Automated Inverted Microscope w/ Perfect Focus & Incubation Chamber (LHRRB 113)

  • Color Imaging

    For Color imaging (brightfield). Uses Station 6 - 80i

  • Equipment consultation

    The core provides technical consultation and support regarding state-of-the-art microscopy and imaging equipment as well as basic and advanced light microscopy techniques.

  • Image Analysis

    "The NIC's focus is to provide help with image acquisition. For help with image analysis, please contact the Image and Data Analysis Core.

    Five Windows PC computer workstations are available in the NIC for image processing and analysis:
    * One (1) computer with a 64-bit processor.
    * Four (4) computers with 32-bit processors.

    Software packages for image processing and analysis:

    * Metamorph image analysis software, including analysis modules (all computers).
    * NIS Elements image analysis software (4 computers, incl. the 64-bit workstation).
    * Autoquant AutoDeBlur deconvolution software (3 computers, incl. 64-bit processor).
    * AutoVisualize 3D renderingsoftware (3 computers, incl. 64-bit processor).
    * Microsoft Excel (all computers).

    Accessing data
    * Data may be accessed through HMS file servers, USB drive, CD, or DVD.
    - It is recommended that you copy your data to the local User Data folder before performing analysis or image processing.
    * These computers can also be used to download data from the servers to USB drive, CD or DVD.
    * Information on connecting to servers can be found on our User Help Sheets page."

  • Imaging and microscopy training

  • Laser Scanning Confocal

    Laser scanning confocal microscopy is available on:
    * Station 3 - Nikon TE2000 w/ C1 Point Scanning Confocal (LHRRB 113)
    * Station 14 - Nikon Ti w/ A1R confocal, Perfect Focus & Stage-top incubation Chamber (LHRRB 113)

  • Live Specimen Imaging

    "Long-term (>4 hours) live cell imaging is available on:

    All long-term scopes are equipped with 37C microscope enclosures, 5% CO2, and auto-focusing. These scopes can be used to image fluorescence, phase and/or DIC.

    Widefield Fluorescence
    * Station 5 - Nikon Ti Automated Inverted Microscope w/ Perfect Focus & 37°C Incubation Chamber (LHRRB 113)
    * Station 9 - Nikon Ti Automated Inverted Microscope w/ Perfect Focus & 37°C Incubation Chamber (LHRRB 113)
    * Station 12 - Nikon Ti Automated Inverted Microscope w/ Perfect Focus & 37°C Incubation Chamber (Goldenson 107)

    Confocal and TIRF
    * Station 1 - Nikon Ti w/ Spinning Disk Confocal, Perfect Focus & 37°C Incubation Chamber (LHRRB 113)
    * Station 15 -Nikon Ti Total Internal Reflection Fluorescence w/ Perfect Focus, 6-line laser launch & 37°C Incubation Chamber (LHRRB 113)


    Short-term (< 4 hours) live cell imaging is available on:

    All short-term microscopes have stage top 37C heaters available for use, and auto-focusing. These scopes can be used to image fluorescence, phase and/or DIC.

    Widefield Fluorescence
    * Station 2 - Nikon Ti w/ Spinning Disk Confocal, Perfect Focus & FRAP/Photoactivation (LHRRB 113)
    * Station 8 - Nikon Ti Inverted Fluorescence Microscope w/ Perfect Focus (LHRRB 113)

    Confocal and TIRF
    * Station 2 - Nikon Ti w/ Spinning Disk Confocal, Perfect Focus & FRAP/Photoactivation (LHRRB 113)
    * Station 4 - Nikon Ti Total Internal Reflection Fluorescence w/ Perfect Focus (Goldenson 107)
    * Station 10- Nikon Ti w/ Perfect Focus, Spinning Disk Confocal & Stage-top Incubation Chamber (Goldenson 107)
    * Station 14 -Nikon Ti w/ A1R confocal, Perfect Focus & Stage-top Incubation Chamber (LHRRB 113)"

  • Microfluidic Perfusion

    We have a CellASIC ONIX microfluidic perfusion system that can be used with most microscopes in the NIC. Users must purchase their own plates from CellASIC to use with the system.

  • Microinjection

    Microinjection can be performed using Station 7 - Microinjection.

  • Microscopy workshops/courses

    "A primary goal of the NIC@HMS is to provide training in basic and advanced light microscopy techniques. The NIC@HMS offers 1-day workshops that are open to members of academic or other non-profit institutions."

    (For rates for commercial institutions, please contact the core director.)

  • Phase Contrast and Differential Interference Contrast (DIC)

    All of our microscope stations have optics for phase contrast and/or DIC.

  • Photo-bleaching, Photo-activation, & Laser Ablation

    Photo-bleaching, Photo-activation, & Laser Ablation techniques can be performed on the following stations:
    * Station 2 - Nikon Ti w/ Spinning Disk Confocal, Perfect Focus, & FRAP/Photoactivation Laser (LHRRB 113)
    * Station 14 - Nikon Ti w/ A1R confocal, Perfect Focus & Stage-top Incubation Chamber (LHRRB 113)

  • Spinning Disk Confocal Microscopy

    Spinning disk confocal microscopy is available on:
    * Station 1 - Nikon Ti w/ Spinning Disk Confocal, Perfect Focus & 37°C/5% CO2 Incubation Chamber (LHRRB 113)
    * Station 2 - Nikon Ti w/ Spinning Disk Confocal, Perfect Focus, & FRAP/Photoactivation Laser (LHRRB 113)
    * Station 10- Nikon Ti w/ Spinning Disk Confocal, Perfect Focus, & Stage Top Incubation Chamber (Goldenson 107)

  • Super-resolution microscopy

    N-STORM is available on: Station 4 - Nikon Ti Total Internal Reflection Fluorescence w/ Perfect Focus

  • Total Internal Reflection Fluorescence (TIRF) microscopy

    Total Internal Reflection Fluorescence (TIRF) microscopy is available on:
    * Station 4 - Nikon Ti Total Internal Reflection Fluorescence w/ Perfect Focus (Goldenson 107)
    * Station 15 - Nikon Ti Total Internal Reflection Fluorescence w/ Perfect Focus, 6-line laser launch & 37°C Incubation Chamber (LHRRB 113)

  • User-based microscopy service

    The Department of Cell Biology, the Department of Systems Biology and the Department of Biochemistry and Molecular Pharmacology (BCMP) at Harvard Medical School subsidize use of the NIC@HMS by members of their departments. Members of these departments therefore have full access to the equipment with no hourly usage fees. The other basic science departments at Harvard Medical School have access to the equipment for a rate of $50 per hour. Prior to using a microscope in the NIC, all users meet with the NIC Director to discuss their imaging plans and decide on the appropriate equipment and approach. All users pay a one-time $200 fee to be trained by an NIC staff member to use a microscope in the NIC. After training, the NIC staff microscopy expertise and guidance continues to be available to all users at no additional charge.

  • Widefield Fluorescence

    Widefield fluorescence of fixed samples is typically performed on
    * Station 6 - Nikon 80i Upright Microscope (LHRRB 113)
    * Station 8 - Nikon Ti Inverted Fluorescence Microscope w/ Perfect Focus (LHRRB 113)


Departmental Web Link(s):

Last updated: 2015-11-19T12:49:00.520-06:00