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Optical Microscopy Core (PCMM @ Children’s Hospital Boston)

Location: 200 Longwood Ave., WAB Rooms 175 and 274A, Boston, MA 02115, 3 Blackfan Circle, 3rd/Fl, Room 3123, Boston, MA 02115

Core Summary:

Confocal and multiphoton are modern fluorescence techniques in microscopy for generating optical sections from live or fixed biological specimens. In general, both techniques employ a point scanning and point detecting design. The confocal microscope achieves point detection by using a confocal pinhole to block off out-of-focus emission from the specimen. The multiphoton microscope, on the other hand, generates intrinsic point emission directly from the in-focus spot, thereby eliminating the need for a confocal pinhole. Subsequent scanning of the entire field of view results in an optical section. Computer reconstruction of serial optical sections, collected at consecutive axial (z) positions, can reveal the spatial localization of cells and tissues (sometime subcellular molecules) in 3D. The ability to see biochemical processes in live cells, in real time, sheds light on the vastly complex molecular world of cells and may allow IDI scientists to identify new targets for drugs that will treat exposure to dangerous toxins and bacteria as well as fight a wide variety of diseases.

Institutions:
  • Boston Children's Hospital
  • Program in Cellular and Molecular Medicine at Children's Hospital (PCMM)
Personnel/Contact Information:

Member: Leung, Harry
Role: Core Manager
Phone: (617) 713-8299
Email

Facilities and Equipment:

Location of Core: 200 Longwood Ave., WAB Rooms 175 and 274A, Boston, MA 02115, 3 Blackfan Circle, 3rd/Fl, Room 3123, Boston, MA 02115

Major Equipment:

  • AutoDeblur
  • Confocal Assistant
  • ImageJ
  • Imaris
  • Lasersharp
  • Marianas SDC system, with Yokogawa CSU22 Spinning Disk
  • Olympus Fluoview FV1000 confocal system
  • Olympus FV1200 MPE multiphoton system
  • SlideBook
  • Volocity
  • VoxBlast
Services:
  • Microscope access service

    "All of the microscopes at the IDI Microscopy Core are shared equipment and are available to both internal and outside users. However, IDI users are given priority."

  • Microscope training service

    "Training is required for use of any Core equipment. In general, five to six hours hands on training will be required for unsupervised operation of most microscopes. A user must show proficiency in operation of a particular instrument before they will be given access to the scheduling calendar to book their own time. For further information and to request training, please contact the Core Manager."


Departmental Web Link(s):

Last updated: 2015-12-04T09:50:25.367-06:00