A searchable database of core laboratory facilities. Core Facilities

Advanced Microscopy Core (JDC)

Director: Bonner-Weir, Susan, Ph.D.

Location: Joslin Diabetes Center, One Joslin Place, Boston, MA 02215

Core Summary:

The Advanced Microscopy Core of Joslin ‘s Diabetes Research Center facilitates the ready availability of sophisticated morphological techniques by providing electron microscopic services, a confocal microscope facility, a laser capture microdissection facility, as well as preparation of frozen or paraffin sections from tissues provided by investigators Additionally we provide protocols, analysis and interpretation.

Since the core is subsidized by the NIH funded DRC, members of the Joslin DRC have priority and a discounted fee-for-service. Use by outsiders will be considered if there is time available, with NIH grant holders given preference.

Institutions:
  • Joslin Diabetes Center
Personnel/Contact Information:

Director: Bonner-Weir, Susan, Ph.D.
Phone: (617) 309-2581
Email

Member: Cahill, Chris
Role: Lab Manager
Email

Member: Pinkhasov, Alevtina
Role: Histology Tech
Email

Facilities and Equipment:

Location of Core: Joslin Diabetes Center, One Joslin Place, Boston, MA 02215

Major Equipment:

  • Arcturus Pixcell II Laser capture microscope and accessories
  • Cryostat
  • Zeiss Laser Scanning microscope (LSM) 710 NLO Live with multiphoton capability and live imaging with inverted lens
  • Zeiss LSM 410 with inverted lens
Services:
  • Confocal microscope access service

    "After training, researchers can use independently with sign up on Intranet."

  • Cryostat sectioning service

    "Frozen blocks of tissue are prepared by the investigator and brought to the core technician with instructions. Unstained and H&E sections and the blocks are returned to the investigator."

  • Histological and morphological services

    "Consultation on histological and morphological analysis and interpretation."

  • Immunochemistry service

    "Immunochemistry on ultrastructural level."

  • Laser capture microdissection service

    "Arcturus Pixcell II Laser capture microscope and accessories. After being trained, researchers use it independently."

  • Paraffin embedding/sectioning service

    "Fixed tissue is prepared in investigator's lab and brought to the core for a weekly scheduled paraffin embedding. Unstained and H&E sections and their blocks are returned to the investigator."

  • Transmission electron microscopic analysis


Departmental Web Link(s):

Last updated: 2014-06-10T18:38:45.288-05:00