A searchable database of core laboratory facilities. Core Facilities

Flow Cytometry Core (JDC)

Director: Wagers, Amy, Ph.D.; Serwold, Thomas F., Ph.D.

Location: Joslin Diabetes Center, One Joslin Place, Boston, MA 02115

Core Summary:

The Flow Cytometry Core Facility provides high speed cell sorting, multi-color fluorescence cell analysis and data interpretation to Joslin Investigators. The aim of this core facility is to provide a comprehensive service with high quality for scientific researchers. The staff at the facility works closely with investigators to develop new techniques to meet research needs. We welcome investigators interested in developing new modes of analysis.

The core is open to everyone. Different pricing for HSCI vs academic non-HSCI and non-academic institutions.

Institutions:
  • Harvard Stem Cell Institute
  • Joslin Diabetes Center
Personnel/Contact Information:

Director: Wagers, Amy, Ph.D.
Phone: (617) 732-2590
Email

Director: Serwold, Thomas F., Ph.D.
Phone: (617) 732-2622
Email

Member: LaVecchio, Joyce
Role: Core Facility Manager
Phone: (617) 309-3450
Email

Member: General Inquiries
Phone: (617) 732-2400
Email

Facilities and Equipment:

Location of Core: Joslin Diabetes Center, One Joslin Place, Boston, MA 02115

Major Equipment:

  • BD 5 laser SORP ARIA
  • BD FACSAria cell sorter
  • BD LSR II analyzer
  • Beckman/Coulter MoFlo cell sorter
  • FlowJo
  • Miltenyi autoMacs separator
  • Miltenyi MACSQuant analyzer
  • Summit
  • Union Biometrica Large Particle BioSorter
Services:
  • Apoptosis analysis service

    "Detection with Annexin V, PI."

  • Calcium flux analysis service

    "Calcium flux analysis is done using Indo-1."

  • Cell cycle analysis service

    "DNA content (PI, 7AAD, Hoechst33342), S-phase detection (BrdU), and CFSE for label dilution analysis of sequential cell divisions."

  • Cell tracking service

    "Cell tracking is done by using CFSE, SNARF, PKh26, PKH67 or PKH2."

  • Fluorescent color analysis/sorting

    15 fluorescent color analysis/sorting on 5 laser BD SORP Aria:
    lasers: 350, 407, 488, 561, and 647

    8 fluorescent color analysis/sorting on3 laser Beckman/Coulter MoFlo:
    lasers: 350, 488 and 647

    12 fluorescent color analysis/sorting on 3 laser BD Aria:
    lasers: 407, 488 and 647

    13 fluorescent color analysis on 4 laser BD LSRII
    laser: 407, 488, 561 and 647

    7 fluorescent color analysis on 3 laser MACSQuant:
    lasers: 407, 488 and 647

  • Gene expression analysis service

    "Gene expression analysis for reporters expressing GFP or GFP derivatives (YFP, CFP, etc.)"

  • High speed cell sorting service

    "Cells can be sorted and separated based on multi-fluorescence analysis on the MoFlo cytometer (> 30,000 cells/sec)."

  • Immunophenotyping service

    "Up to 6 fluorescent colors analysis with multi-laser excitation on the MoFlo and up to 4-color analysis with single laser excitation. All common fluorochromes (U.V. to infrared)."

  • Single-cell sorting service

    We provide single-cell sorting for cloning and DNA/RNA analysis.


Departmental Web Link(s):

Last updated: 2012-08-08T09:37:44.984-05:00