Flow Cytometry Core (JDC)
Director: Wagers, Amy, Ph.D.; Serwold, Thomas F., Ph.D.
Location: Joslin Diabetes Center, One Joslin Place, Boston, MA 02115
The Flow Cytometry Core Facility provides high speed cell sorting, multi-color fluorescence cell analysis and data interpretation to Joslin Investigators. The aim of this core facility is to provide a comprehensive service with high quality for scientific researchers. The staff at the facility works closely with investigators to develop new techniques to meet research needs. We welcome investigators interested in developing new modes of analysis.
The core is open to everyone. Different pricing for HSCI vs academic non-HSCI and non-academic institutions.
Director: Wagers, Amy, Ph.D.
Phone: (617) 732-2590
Director: Serwold, Thomas F., Ph.D.
Phone: (617) 732-2622
Role: Core Facility Manager
Phone: (617) 732-2400
|Facilities and Equipment:|
Location of Core: Joslin Diabetes Center, One Joslin Place, Boston, MA 02115
- 3-Laser BL2 BD FACSAria II u
- 5-laser BD SORP ARIA
- BD FACSAria cell sorter
- BD LSRFortessa
- BD LSR II analyzer
- Beckman/Coulter MoFlo cell sorter
- Cellometer Vision Trio Cellprofiler
- ELISPOT reader
- Hemavet 950 FS
- Miltenyi autoMacs separator
- Miltenyi MACSQuant analyzer
- Union Biometrica Large Particle BioSorter
- Apoptosis analysis service
"Detection with Annexin V, PI."
- Automatic cell counting
This service uses Cellometer and Hemavet for cell counting.
- Calcium flux analysis service
"Calcium flux analysis is done using Indo-1."
- Cell analysis
Uses the BD LSR-II, Miltenyi Biotec MACSQuant and BD LSRFortessa to perform fluorescent color analysis.
- Cell cycle analysis service
"DNA content (PI, 7AAD, Hoechst33342), S-phase detection (BrdU), and CFSE for label dilution analysis of sequential cell divisions."
- Cell sorting
Service uses MoFlo Legacy, SORP BD FACSAria and BD FACSaria Hu for cell sorting.
- Cell tracking service
"Cell tracking is done by using CFSE, SNARF, PKh26, PKH67 or PKH2."
- Data analysis
For flow cytometry data analysis, the Core provides 3 FlowJo dongles that can be signed out from the core on a weekly basis.
- EliSPot Reader
Scans and evaluates a wide range of microtiter plate-based bioassays, in particular, ELISPOT assays.
- Fluorescent color analysis/sorting
15 fluorescent color analysis/sorting on 5 laser BD SORP AriaII:
lasers: 355, 407, 488, 561, and 640
8 fluorescent color analysis/sorting on3 laser Beckman Coulter MoFlo:
lasers: 355, 488 and 640
12 fluorescent color analysis/sorting on 3 laser BD Aria IIu:
lasers: 407, 488 and 640
15 fluorescent color analysis on 4 laser BD LSRII
laser: 407, 488, 561 and 640
18 fluorescent color analysis on 5 laser BD LSRFortessa
laser: 407,445, 488, 561 and 638
7 fluorescent color analysis on 3 laser MACSQuant:
lasers: 407, 488 and 647
- Gene expression analysis service
"Gene expression analysis for reporters expressing GFP or GFP derivatives (YFP, CFP, etc.)"
- Large object sorting
Service uses the Complex Object Parametric Analyzer and Sorter (COPAS) BioSort system to sort objects ranging from 40-250 microns.
- Magnetic cell enrichment
This service uses AutoMACS to provide high yield enrichment of rare cell populations via magnetic selection, which helps to reduce per user sort times by enabling better pre-sort enrichment of target cells.
- Single-cell sorting service
We provide single-cell sorting for cloning and DNA/RNA analysis.
|Departmental Web Link(s):|