Confocal Microscope
Director: Pradeep Bhide, Ph.D.
Location: Building 149 13th Street, Room 6028, Charlestown
Core Functions: This Core consists of a Zeiss LSM 5 Pascal laser confocal microscope. The Zeiss RGB vario laser module consists of argon laser (458/488/514 nm) and two helium-neon lasers (543 and 633 nm). A Zeiss Axiovert 200 fully motorized light microscope is available with fluorescence, bright-field, phase-contrast and Nomarski (DIC) capabilities. Image acquisition and analyses are performed using Zeiss LSM 5 Pascal Confocal Microscopy Software (Release 3.2) on 2 workstations. Zeiss Physiology software is available also. Live cell imaging is available using a Zeiss temperature controller with custom chamber and heating stage.
| Categories and Institutions: |
- Categories
- Cellular Imaging and Microscopy
| - Institutions
- Massachusetts General Hospital
|
| Personnel/Contact Information: |
Director: Pradeep Bhide, Ph.D.
Phone: 617-726-5763
Fax: 617-726-6656
Email
Member: Igor Bagayev
Role: Research Technician
Phone: 617-724-0588
Fax: 617-726-5677
Email
| Facilities and Equipment: |
Location of Core: Building 149 13th Street, Room 6028, Charlestown
Major Equipment:
- Zeiss Axiovert 200 fully motorized light microscope
- Zeiss LSM 5 Pascal Confocal Microscopy Software (Release 3.2) on 2 workstations
- Zeiss LSM 5 Pascal laser confocal microscope
- Zeiss Physiology software
- Zeiss RGB vario laser module consists of argon laser (458/488/514 nm) and two helium-neon lasers (543 and 633 nm)
- 3-Dimensional reconstruction
- Double- and triple-labeling experiments
- Dynamic events in live cells and tissues
- High-resolution images (2048 x 2048 pixels)
- Intracellular ion concentration
- Intracellular transport mechanism
- Localized scans and FRAP (fluorescence recovery after photo-bleaching)
- Quantitative information for each channel
To access the core, please contact Igor Bagayev at (617) 724-0588 or by e-mail
http://www.massgeneral.org/ncs/neuro_core_ConfocalMicroscope.htm
Last updated: 2009-02-03T22:53:00Z
