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Confocal Microscope Core (MGH)

Director: Bragg, Christopher, Ph.D.

Location: Building 149, 13th Street, Room 6025, Charlestown, MA 02129

Core Summary:

This Core consists of a Zeiss LSM 5 Pascal laser confocal microscope with a Zeiss RGB vario laser module and Nikon C1 Confocal/TIRF System with 3 PMT. A Zeiss Axiovert 200 fully motorized light microscope is available with fluorescence, bright-field, phase-contrast and Nomarski (DIC) capabilities. Image acquisition and analyses are performed using Zeiss LSM 5 Pascal Confocal Microscopy Software (Release 3.2) on 2 workstations. Zeiss Physiology software is available also. Live cell imaging is available using a Zeiss temperature controller with custom chamber and heating stage.
The Nikon C1 Confocal/TIRF System fully motorized Nikon Eclipse Ti microscope is available with fluorescence, bright-field and TIRF capabilities. Imaging acquisition and analyses are performed using EZ-C1 and NIC-Elements Software.

Institutions:
  • Massachusetts General Hospital
Personnel/Contact Information:

Director: Bragg, Christopher, Ph.D.
Phone: (617) 643-5754
Email

Member: Bagayev, Igor
Role: Senior Research Technologist
Phone: (617) 724-0588
Email

Facilities and Equipment:

Location of Core: Building 149, 13th Street, Room 6025, Charlestown, MA 02129

Major Equipment:

  • Melles Griot Confocal/TIRF laser module
  • Nikon C1 Confocal/TIRF System with 3 MPT
  • Nikon Confocal Microscope C1 EZ-C1 Software
  • Nikon Eclipse Ti fully motorized microscope
  • Nikon NIS-Elements Advaced Research Software
  • Zeiss Axiovert 200 fully motorized light microscope
  • Zeiss LSM 5 Pascal laser confocal microscope
  • Zeiss LSM Image Browser Software
  • Zeiss Physiology
  • Zeiss RGB vario laser module
Services:
  • 3-dimensional image reconstruction

  • Acquisition of high-resolution images: 2048 x 2048 pixels

  • Double- and triple-labeling experiments

  • Imaging dynamic events in live cells and tissues

  • Intracellular ion concentration determination

  • Intracellular transport mechanism visualization and analysis service

  • Localized scans and FRAP (fluorescence recovery after photo-bleaching) service

  • Single molecule visualization, allowing dynamic observation and functional analyses of both in vivo and living cells

  • Total internal reflection fluorescence experiments (TIRF)


Departmental Web Link(s):

Last updated: 2012-07-16T09:03:54.260-05:00