A searchable database of core laboratory facilities. Core Facilities

Flow Cytometry Core Facility (MGH)

Director: Preffer, Fred, Ph.D.

Location: 13th Street, Building 149, Room 5.226, Charlestown Navy Yard, and 185 Cambridge Street, Room 3.434, Simches Building, Boston, MA 02114

Core Summary:

The cell sorting and analysis lab provides access to the most sophisticated flow cytometers presently available. The core is able to analyze and physically separate cells based upon features detectable with fluorescently labeled monoclonal antibodies. The Amnis technology combines flow with fluorescence and bright field image-based analysis.

This facility has two locations: the Simches site and the Charlestown Navy Yard (CNY) site.

Institutions:
  • Massachusetts General Hospital
Personnel/Contact Information:

Director: Preffer, Fred, Ph.D.
Phone: (617) 726-7481
Email

Member: Mylvaganam, Ravi, Ph.D.
Role: Senior Research Technician, CNY Site
Phone: (617) 643-6771
Email

Member: Luo, Christina
Role: Research Technician, CNY Site
Phone: (617) 643-6771
Email

Member: Mordecai, Scott
Role: Core Lab Technology Scientist, Amnis Imaging Flow Cytometry, Simches Site
Phone: (617) 726-1683
Email

Member: Dombkowski, David
Role: Senior Research Technician, Simches Site
Phone: (617) 726-1683
Email

Member: General Inquiries
Phone: (617) 726-1683, (617) 726-7481
Email

Facilities and Equipment:

Location of Core: 13th Street, Building 149, Room 5.226, Charlestown Navy Yard, and 185 Cambridge Street, Room 3.434, Simches Building, Boston, MA 02114

Major Equipment:

  • Charlestown: SORP 4 Laser BD FACSAria II Cell Sorter
  • Charlestown: SORP 4 Laser BD Fortessa
  • Charlestown: SORP 4 Laser BD LSR II
  • Charlestown: SORP 5 Laser BD FACSAria Fusion Cell Sorter, BSL2+
  • Charlestown: SORP 5 Laser BD Fortessa
  • MGH/Boston: Amnis ImageStreamx mkII Imaging Flow Cytometer
  • MGH/Boston: SORP 5 Laser BD Digital Vantage SE Cell Sorter
  • MGH/Boston: SORP 8 Laser BD FACSAria Fusion Cell Sorter, BSL2+
  • MGH/Boston: SORP 8 Laser BD LSR
Services:
  • Flow-based imaging analysis

  • Flow cytometric analysis and cell sorting

    State-of-the-art multi-parametric flow cytometric analysis and cell sorting available at both Simches and Charlestown Navy Yard locations.


Departmental Web Link(s):

Last updated: 2015-12-07T15:37:16.944-06:00