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PMB Microscopy Core (MGH)

Director: Brown, Dennis

Location: 185 Cambridge Street, MGH Simches Research Building, 8th Floor, Boston, MA 02114

Core Summary:

The Microscopy Core of the Program in Membrane Biology (PMB) at MGH is equipped and staffed to provide a wide range of services to investigators from MGH and the Boston area scientific community in the area of light and electron microscopy. The Core is housed on the 8th floor of the Simches Research Center at MGH, and is directed by Dr. Dennis Brown, Ph.D. The Core will provide services ranging from complete performance of the technical procedures required, to full training of personnel. Among the techniques available are laser scanning confocal microscopy, spinning disk confocal microscopy, tissue fixation, sectioning, immunostaining and conventional immunofluorescence microscopy, image analysis, and all aspects of electron microscopy including immunogold staining. The Core facility provides a unique and important resource for members of the scientific community as they approach a variety of research problems that call for these advanced microscopy techniques.

Members of BADERC are supported at three levels by this Core. First, short-term or preliminary studies can be performed by Core technicians who are experts in these areas, with little or no hands on input from the PI or colleagues. Second, the lab of the PI can perform part of the work, such as initial cell/tissue preparation or fixation, and then the Core will complete the study. In many cases, investigators from the BADERC lab spend some time in the Core facility being instructed in some of these procedures. Finally, the Core personel will train designated individuals from BADERC labs to become independent in the procedure of choice if the procedure will be established in the long term in a given lab. Thus, all steps from tissue fixation, sectioning, immunostaining and microscopy (including confocal microscopy) will be demonstrated to designated individuals. This usually requires spending at least two weeks in the Core facility. In such cases, the Core equipment is then made available to the BADERC lab for future studies as and when required.

Thus, the Microscopy Core has a variety of menus to fit the microscopy/immunocytochemistry needs of most PIs. Interaction usually involves a preliminary discussion with the Core director and the Core technician who will be involved in the work. Intellectual and practical input are then tailored to fit individual requirements.

Institutions:
  • Massachusetts General Hospital
Personnel/Contact Information:

Director: Brown, Dennis
Phone: (617) 726-5665
Email

Member: Tyszkowski, Robert
Role: Senior Lab Technologist
Phone: (617) 724-9694
Email

Member: McKee, Mary
Role: Senior Lab Technologist
Phone: (617) 726-3696
Email

Member: General Inquiries
Phone: (617) 726-5665
Email

Facilities and Equipment:

Location of Core: 185 Cambridge Street, MGH Simches Research Building, 8th Floor, Boston, MA 02114

Major Equipment:

  • BioRad/Zeiss Radiance 2000 confocal microscope
  • IPLab
  • JEOL 1011 electron microscope
  • Leica APS freeze-substitution system
  • Leica FCS ultracryomicrotome
  • Leica microtome
  • Macintosh G4-based computer workstation
  • Microm cryostat
  • MMI CellCut laser microdissection system
  • Nikon 80i upright epifluorescence microscope with Hamamatsu Orca CCD camera
  • Nikon E800 Eclipse upright epifluorescence microscope with Hamamatsu Orca CCD camera
  • Nikon TE300 inverted microscope with wide-field optics and Hamamatsu Orca CCD camera
  • NikonTotal internal reflection fluorescence microscope system
  • Olympus BH2 upright microscope with Spot Insight color CCD camera
  • Olympus double-headed light microscope
  • PerkinElmer LCI spinning disk confocal microscope
  • Power Mac G5 based workstation
  • Reichert ultramicrotome
  • Volocity
Services:
  • Computer based image analysis and quantification

  • Confocal microscopy service

    In some cases, investigators will provide their own stained tissues/cell for imaging, while in others the Core will perform all services from fixation and sectioning, to staining and imaging (when applicable).

  • Conventional electron microscopy service

    In some cases, investigators will provide their own stained tissues/cell for imaging, while in others the Core will perform all services from fixation and sectioning, to staining and imaging (when applicable).

  • Conventional immunofluorescence microscopy service

    In some cases, investigators will provide their own stained tissues/cell for imaging, while in others the Core will perform all services from fixation and sectioning, to staining and imaging (when applicable).

  • Development for Core enhancement service

    Dr. Dennis Brown and the advisory committee will continually explore new techniques to be offered as part of the core service.

  • EM immunogold labeling service

    In some cases, investigators will provide their own stained tissues/cell for imaging, while in others the Core will perform all services from fixation and sectioning, to staining and imaging (when applicable).

  • Live-cell imaging service

    In some cases, investigators will provide their own stained tissues/cell for imaging, while in others the Core will perform all services from fixation and sectioning, to staining and imaging (when applicable).

  • Microscopy consultation services

    Dr. Dennis Brown and the Core technologists will be available for consultation in the use of the different microscopy techniques, and to provide technical advice, such as the different fixation techniques that enable antibodies to detect their antigens.

  • Microscopy training services

    The core technicians will also provide training to high frequency users who wish to invest the substantial time necessary for learning to use the equipment proficiently.

  • Technical microscopy service

    The core will perform conventional immunofluorescence microscopy, confocal microscopy, live-cell imaging, conventional electron microscopy and EM immunogold labeling, tissue sectioning, tissue fixation, and computer based image analysis and quantification. In some cases, investigators will provide their own stained tissues/cell for imaging, while in others the Core will perform all services from fixation and sectioning, to staining and imaging.

  • Tissue fixation

    In some cases, investigators will provide their own stained tissues/cell for imaging, while in others the Core will perform all services from fixation and sectioning, to staining and imaging (when applicable).

  • Tissue sectioning service

    In some cases, investigators will provide their own stained tissues/cell for imaging, while in others the Core will perform all services from fixation and sectioning, to staining and imaging (when applicable).


Departmental Web Link(s):

Last updated: 2011-06-14T09:58:51.511-05:00