New England Regional Center of Excellence - Live-cell Imaging Resource
Location: Immune Disease Institute on the Harvard Medical School campus in Boston, MA
| Core Summary: |
Live-cell imaging is a powerful method that enables investigators to observe cellular trafficking events with a high degree of spatial and temporal resolution, including visualization of individual bacteria or viral particles engaged in the process of infecting eukaryotic cells. A quantitative description of bacterial and viral invasion mechanisms can be obtained through such approaches. NERCE has provided funding to support the use of live-cell imaging for any New England investigator studying NIAID priority pathogens and agents of emerging infectious disease. This resource is located at the Immune Disease Institute on the Harvard Medical School campus in Boston, MA.
| Categories and Institutions: |
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| Personnel/Contact Information: |
Member: Eric Marino
Department: Immune Disease Institute
Role: Technical Inquiries
Phone: 617-278-3014
Fax: 617-278-3131
Email
Member: Gerald A. Beltz, Ph.D.
Role: Associate Director of Research
Phone: 617-432-5520
Fax: 617-432-5506
Email
| Facilities and Equipment: |
Location of Core: Immune Disease Institute on the Harvard Medical School campus in Boston, MA
Major Equipment:
- Zeiss 200M microscopes
| Services: |
- 6-node multiprocessor system for real-time 3D image deconvolution
- Comprehensive one-on-one training
- Integrated RAID server with tape backup for image storage
- Save images in common file formats for use with other analysis or presentation software
- SlideBook acquisition and 3D analysis software
- Two completely motorized inverted Zeiss 200M microscope
* Fast image acquisition in two and three dimensions as a function of time, location and multiple probes (6D) * Spinning disk head offers confocality and faster acquisition with less photo-toxicity than Laser Scanning Confocal Microscopes * Total Internal Reflection Fluorescence (TIRF) capability with simultaneous dual wavelength illumination for molecular dynamics * Computer-controlled spherical aberration correction (SAC) provides geometrically and intensely uniform 3D acquisition * FRAP/photo-activation * Fully enclosed environmental control (37 °C, 5% CO2) creating an axially stable system over time and a cell friendly environment * 10x, 20x, 40x dry and 40x, 63x 100x high N.A. oil immersion objectives * Broad spectrum of excitation wavelengths from 350nm – 660nm and emission from 450nm – 670nm
| Departmental Web Link: |
http://nerce.med.harvard.edu/livecell.html