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HSCI-CRM Flow Cytometry Core Facility @ MGH

Director: Scadden, David, M.D., Ph.D

Location: Richard B. Simches Research Center, Center for Regenerative Medicine, Massachusetts General Hospital, 185 Cambridge Street, CPZN - Room 4310, Boston, MA 02114

Core Summary:

The HSCI-CRM Flow Cytometry Core Facility seeks to provide high quality, accessible cytometry sorting and analysis services to laboratories in the Center for Regenerative Medicine, Harvard Stem Cell Institute, and MGH research communities at an affordable rate. The Core's equipment and highly trained operators provide an advanced level of sorting and analysis services to its investigators. Additionally, the Core is dedicated to training users on all aspects of flow cytometry, including basic theory, information about specific applications, and critical interpretation of sorting results. The Core's four full-time staff members strive to ensure that each investigator's visit benefits their individual experiments to the greatest extent possible. To ensure the best availability to all users, the Core offers an online scheduling system, and provides extended sorting hours until late evening.

HSCI faculty have reduced pricing for use of the Core's services.

Institutions:
  • Harvard Stem Cell Institute
  • Massachusetts General Hospital
Personnel/Contact Information:

Director: Scadden, David, M.D., Ph.D
Phone: (617) 726-5615
Email

Member: Handley, Maris
Email

Member: General Inquiries
Phone: (617) 643-0790
Email

Facilities and Equipment:

Location of Core: Richard B. Simches Research Center, Center for Regenerative Medicine, Massachusetts General Hospital, 185 Cambridge Street, CPZN - Room 4310, Boston, MA 02114

Major Equipment:

  • BD FACSAria II
  • BD FACSAria IIu in biosafety cabinet
  • BD FACSAria SORP in biosafety cabinet
  • BD FACSDiva
  • BD LSR II analyzer
  • FlowJo
  • Miltenyi AutoMACS Magnetic Cell Sorter
  • ModFit LT
  • Several computers for FlowJo, ModFit LT, and FACSDiva offline analysis
Services:
  • FACSAria Self-run sorting

  • FACSAria sorting

  • LSRII self-run analysis service

  • Offline assisted or self-performed analysis

    We have several computers available for independent offline data analysis. Two Apple iMacs are in the main flow room; both have FlowJo installed, and one also has ModFitLT for cell cycle analysis. There is one additional Apple iMac in the CRM computer room for FlowJo and ModFit analysis. There is also a WindowsXP computer configured with FACSDiva 6.1 for further analysis of experiments collected on the LSRII or FACSArias. We provide training in FlowJo and ModFit LT for interested users. You may book time with us to prepare data for publication.

    FlowJo software is free to download from flowjo.com but requires a hardware license. We have several FlowJo dongles available to lend out to users to analyze their data on their own computers. We request users return a dongle when they are not actively using it to ensure there are dongles available for all users.

  • Self-run analyzer or sort training

    Training is available for using the various flow cytometers.


Departmental Web Link(s):

Last updated: 2017-08-28T12:04:52.713-05:00