A searchable database of core laboratory facilities. Core Facilities

Proteomics Center at Boston Children's Hospital

Director: Steen, Hanno, Ph.D.

Location: Proteomics Center at Boston Children's Hospital, Department of Pathology, Enders 1155, 320 Longwood Ave., Boston, MA 02115

Core Summary:

The Proteomics Center at Children's Hospital Boston offers the most up-to-date proteomics equipment currently available. This includes the latest equipment for protein separation and several state-of-the-art mass spectrometers, which detect and quantify proteins in a sample and measure them to determine their structure and characteristics.

Institutions:
  • Boston Children's Hospital
Personnel/Contact Information:

Director: Steen, Hanno, Ph.D.
Phone: (617) 919-2629
Email

Member: Ahmed, Saima, B.S.
Role: Lab Manager, Proteomics Center at Children's Hospital Boston
Phone: (617) 919-2757
Email

Facilities and Equipment:

Location of Core: Proteomics Center at Boston Children's Hospital, Department of Pathology, Enders 1155, 320 Longwood Ave., Boston, MA 02115

Major Equipment:

  • Automated LC / ESI Mass Spectrometer
  • FT-ICR mass spectrometer
  • MALDI Mass Sepctrometer
Services:
  • Data interpretation and consulting by operator/staff

    Includes: data Interpretation and consulting, per hour.

  • Enzymatic digests service

  • Gel handling service

    "Documenting gel image, excising gel bands (up to 20 gel bands)."

  • ICAT (Data Acquisition and Analysis)

    Isotope-coded affinity tags (ICATs) analysis

  • ITRAQ (Data Acquisition and Analysis)

    Isobaric tags for relative and absolute quantitation (iTRAQ) analysis

  • Protein identification from complex mixtures (in solution) service

    "Includes reduction, alkylation, dialysis, 45 min instrument time and data analysis."

  • Protein identification from Coommassie stained gel

    Includes in-gel digestion, extraction, analysis and data interpretation.

  • Protein identification from silver stained gel

    Includes in-gel digestion, extraction, analysis and data interpretation.

  • Protein Modification analysis

  • Running SDS-PAGE service

    "Per gel, samples provided in sample buffer by customer; includes running gel, staining, documenting and excising up to 40 bands)."

  • SILAC (Data Acquisition and Analysis)

    Stable isotope labeling by amino acids in cell culture (SILAC) analysis


Departmental Web Link(s):

Last updated: 2015-08-25T11:45:55.471-05:00