A searchable database of core laboratory facilities. Core Facilities

RNA Interference Screening Facility (DFCI)

Director: Hahn, William, M.D., Ph.D.; Schinzel, Anna, Ph.D.

Location: DANA 15, 44 Binney St., Boston, MA 02115

Core Summary:

The RNAi Screening Facility provides investigators with access to RNAi reagents and high-throughput screening technology. Lentiviral-based RNAi constructs against the human and mouse genomes are provided by the RNAi Consortium of the Broad Institute, and can be used to transduce a wide range of target cells, including primary and non-dividing cells. The Facility provides reagents, equipment, and technical expertise to design, optimize, and perform cell-based, arrayed lentiviral RNAi screens. Additionally, lentiviral RNAi constructs against individual genes can be requested.

Institutions:
  • Dana-Farber Cancer Institute
Personnel/Contact Information:

Director: Hahn, William, M.D., Ph.D.
Phone: (617) 632-2641
Email

Director: Schinzel, Anna, Ph.D.
Phone: (617) 582-7649
Email

Facilities and Equipment:

Location of Core: DANA 15, 44 Binney St., Boston, MA 02115

Major Equipment:

  • BioTek Microfill microplate dispenser
  • MDS SpectraMax plate reader
  • Perkin-Elmer EnVision multi-label plate reader
  • Perkin-Elmer Janus mini liquid handling robot
Services:
  • Custom RNAi screening

    Custom RNAi screening sets are available upon request.

  • Genome-scale screening of lentiviral human and mouse shRNA libraries

    "Lentiviral-based RNAi constructs against the human and mouse genomes are provided by the RNAi Consortium of the Broad Institute, and can be used to transduce a wide range of target cells, including primary and non-dividing cells. The Facility provides reagents, equipment, and technical expertise to design, optimize, and perform cell-based, arrayed lentiviral RNAi screens."

  • Tissue culture facility access

    Use of a lentiviral BL2+ tissue culture facility is available for authorized, trained users.


Departmental Web Link(s):

Last updated: 2011-06-22T10:08:29.886-05:00