A searchable database of core laboratory facilities. Core Facilities

Drosophila RNAi Screening Center (HMS)

Director: Mohr, Stephanie, Ph.D.

Location: New Research Building, Room 336, 77 Avenue Louis Pasteur, Boston, MA 02115

Core Summary:

The DRSC facilitates genome-wide and related Drosophila cell-based and in vivo screening at our state-of-the-art facility. We prove reagents, protocols and consultation throughout the screening process, including help with production of custom RNAi libraries; assay development, optimization and automation; data acquisition; data analysis and integration; and planning of follow-up assays.

Institutions:
  • Harvard Medical School
Personnel/Contact Information:

Director: Mohr, Stephanie, Ph.D.
Phone: (617) 432-5626
Email

Member: General Inquiries
Phone: (617) 432-5626
Email

Facilities and Equipment:

Location of Core: New Research Building, Room 336, 77 Avenue Louis Pasteur, Boston, MA 02115

Major Equipment:

  • Agilent Velocity Bravo liquid handling robot
  • Formulatrix Mantis liquid handling robot
  • GE IN Cell 6000 automated confocal imaging system
  • LiCor Aerius fluorimeter
  • Molecular Devices Paradigm luminometer and fluorimeter
  • Perkin Elmer Multiprobe liquid handling robot
Services:
  • Bioinformatics and reagent support for Drosophila “RNAi rescue” using a cross-species approach

    Bioinformatics and reagent support for Drosophila “RNAi rescue” using a cross-species approach (i.e. fosmid clones from a non-melanogaster species that confer gene activity but evade the RNAi reagent), facilitating the ‘gold standard’ rescue test for gene specificity of cell-based or in vivo RNAi phenotypes

  • Bioinformatics support for functional genomics analyses in Drosophila and other species

    Bioinformatics support for functional genomics analyses in Drosophila and other species, including identification of orthologs among common model organisms (yeast, worm, zebrafish, Drosophila) and humans, on-line tools and expert advice on Drosophila RNAi reagent design, identification of Drosophila orthologs of human disease genes, mining public RNAi screen data, analyzing RNAi screen data, etc.

  • Custom synthesis of double-stranded RNAs for Drosophila cell-based RNAi

    Custom synthesis of double-stranded RNAs for Drosophila cell-based RNAi in 96- or 384-well format, based on a researcher-submitted gene list, including re-array and aliquotting of the library into assay-read plates.

  • Drosophila genome-wide and focused cell-based RNAi libraries

    Drosophila genome-wide and focused cell-based RNAi libraries (full-genome screening library, follow-up library targeting unique regions as compared with the screening library, and several sub-libraries, including a kinases and phosphatases library, transcription factors and related DNA binding library, transmembrane domain-containing library, and more). Available in assay-ready formats for 384-well micro-well plate-based cell-based assay screening of Drosophila cultured or primary cells. The libraries are designed and generated in-house.

  • Production of transgenic Drosophila for in vivo tissue- or stage-specific RNAi

    Production of transgenic Drosophila for in vivo tissue- or stage-specific RNAi, based on our in-house developed vectors for Ga4-UAS system expression of shRNAs, with new genes added to the production list based on community nominations

  • Support for a wide range of cell-based assays using Drosophila or mammalian cells

    Support for a wide range of cell-based assays using Drosophila or mammalian cells (fixative-treated cells only; no live mammalian cells), including luminescence, fluorescence and high-content imaging (see Instrumentation)


Departmental Web Link(s):

Last updated: 2017-03-09T14:26:53.074-06:00