A searchable database of core laboratory facilities. Core Facilities

Flow Cytometry Core Laboratory (BWH)

Location: 77 Avenue Louis Pasteur, NRB 435E, Boston, MA 02115

Core Summary:

This lab's mission is "to provide high standard cell sorting and analysis services to the research community at the Brigham and Women’s Hospital and the Longwood Medical area."

Institutions:
  • Brigham and Women's Hospital
Personnel/Contact Information:

Member: Losyev, Grigoriy, M.D.
Role: Associate in Medicine
Phone: (617) 525-4011
Email

PI: Liao, Ronglih, Ph.D.
Role: Associate Professor of Medicine
Phone: (617) 525-4854
Email

Member: General Inquiries
Email

Facilities and Equipment:

Location of Core: 77 Avenue Louis Pasteur, NRB 435E, Boston, MA 02115

Major Equipment:

  • BD FACSAria Special Order 11 color sorter
Services:
  • Calcium flux measurements service

    Using Indo-1.

  • Cell sorting service

    Service includes: single cell sorting (cloning) into 96-well and 384-well plates, sorting (up to 10 colors) of multicolor immunophenotyped samples, sorting and analysis of GFP, YFP, mTomato, gene expression proteins, sorting stem cell side populations using Hoechst, and sorting up to 4 populations at once into Eppendorf, 5 ml or 15 ml tubes.

  • DNA analysis service

    Uses standard dyes (Hoeschst, DAPI, PI, 7-AAD, etc.).

  • Sorting or analysis with operator service

    "Specialties: Multicolor immunophenotyping. 11 colors including Qdots by customer choice; DNA analysis with Hoechst 33342 and 33358; DAPI, PI, YoYo1, DRAQ5; Analysis of Side Population with Hoechst 33342 and cells surface staining; GFP, YFP, td-Tomato; High through put flow cytometry combined with Interllycyt system. Utilizing multiplex Luminex bead assays."

    References:
    http://sites.google.com/site/bwhflowcytometry/services


Departmental Web Link(s):

Last updated: 2011-04-28T14:23:56.438-05:00