Confocal and Light Microscopy Core
Director: Lisa Cameron, Ph.D.
Location: Jimmy Fund Building, 220A,220B, 44 Binney Street, Boston, MA 02115
| Core Summary: |
The Confocal and Light Microscopy Core provides state-of-the-art imaging microscopy technology for fixed and live cells and tissues along with scientific and technical expertise to assist in experimental design and optimal image collection. Imaging techniques available include standard fluorescence, live-cell long term imaging, confocal, TIRF, FRET, and spectral imaging. Available equipment includes a spinning disk confocal microscope, a Leica SP5 laser scanning confocal with a white light laser, and an inverted widefield fluorescence microscope. Other services include training on all equipment, consultation about various imaging techniques and assistance with image analysis. In addition, staff are available to assist with troubleshooting regarding microscopes in individual research labs. All services are available on a fee basis, and equipment is available for either assisted use or unassisted use after training by core staff.
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| Personnel/Contact Information: |
Director: Lisa Cameron, Ph.D.
Phone: 617-582-8824
Email
| Facilities and Equipment: |
Location of Core: Jimmy Fund Building, 220A,220B, 44 Binney Street, Boston, MA 02115
Major Equipment:
- Spinning Disk uses Yokogawa’s microlens technology along with a Nipkow disk
- Widefield - Nikon inverted live-cell imaging system
| Services: |
- Consultation
The Director is available to discuss your project and imaging needs, help troubleshoot sample preparation, optimize the imaging equipment, or assist with equipment in your own lab.
- Help in Your Lab
If you need help with microscopy equipment that you have in your lab, please contact the Core Director. Services available in your own lab include: * Bulb replacement * Koehler illumination tutorial * Microscope / objective cleaning * Diagnose a problem with your microscope
- Microscope Use
The DFCI Confocal and Light Microscopy Core Facility has several types of microscopy equipment available for use. We have a widefield (non-confocal) inverted microscope that has both transmitted light and epi-fluorescence illumination which can be used for both fixed cell fluorescence imaging or live-cell imaging. In addition, a spinning disk confocal microscope on an inverted Nikon microscope is available. We plan to purchase a laser scanning confocal / multi-photon system in the coming fiscal year. All systems will be available for assisted or unassisted use.
- Training
In order to use core facility equipment on your own, each user must complete training by the Core Director for each microscope system s/he would like to use. We encourage users to seek microscope training, rather than relying on assisted use, as this will help to educate the user about how the equipment works and how to best prepare their scientific samples for microscopy imaging.
| Departmental Web Link: |
http://microscopy.dfci.harvard.edu