Imaging Microscopy Core
Director: Susan J. Hagen, Ph.D.
| Core Summary: |
The Microscopy Core Facility at the Beth Israel Deaconess Medical Center (BIDMC) has 2 active microscopy services. First, the Electron Microscopy Core provides service, technical assistance, and instrumentation for electron microscopy techniques. Second, the Confocal Microscopy Service provides service and technical assistance for immunostaining, conventional and live-cell confocal microscopy, light (photodocumentation) and fluorescence microscopy, digital image acquisition and processing, and digital and conventional darkroom printing. All work is done on a fee-for-service basis to recover costs for equipment upkeep and technical help. Priority use is given to BIDMC investigators, but outside users are accepted.
| Categories and Institutions: |
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| Personnel/Contact Information: |
Director: Susan J. Hagen, Ph.D.
Phone: 617-667-5308
Email
Member: Andrea Calhoun, BS
Role: EM Supervisor
Phone: 617-667-4188
Fax: 617-975-5562
Email
Member: Lay-Hong Ang, PhD
Role: Confocal Supervisor
Phone: 617-667-5373
Fax: 617-975-5562
Email
| Facilities and Equipment: |
Major Equipment:
- Agfa-Gevaert print processor
- Balzers MED020 vacuum evaporator
- Durst and Omega enlargers
- Improvision Velocity software on a Dell workstation, and Fuji pictography color printer
- JEOL 1200EX transmission electron microscope
- Nikon E800 light/fluorescence microscope
- Ultracut E ultramicrotome and Ultracut S cryo-ultramicrotome
- Zeiss LSM510 META live-cell confocal system
- Zeiss LSM510 META confocal system
| Services: |
- Full range of standard microscopic services
Including tissue preparation and embedding, thick and thin sectioning of tissues, frozen sectioning for light, fluorescence, and/or confocal microscopy, positive and negative staining, immunocytochemistry, immunohistochemistry, immunofluorescence and immunoperoxidase labeling of tissue sections, development and printing of photographic material.
- Our transmission electron microscope is available for use by any researcher at the BIDMC who is trained or is willing to be trained in electron microscopy.
| Departmental Web Link: |
http://bidmc.org/Research/CoreFacilities/ImagingMicroscopyCore.aspx