A searchable database of core laboratory facilities. Core Facilities
Alert: The Harvard Catalyst website will be unavailable due to system maintenance from Friday (02/24) 10pm to Saturday (02/25) 1pm.

Confocal Imaging Core (BIDMC)

Director: Hagen, Susan J., Ph.D.

Location: 330 Brookline Avenue, Dana Building, Room 814, Boston, MA 02215

Core Summary:

The function of the Confocal Core is to provide services in confocal microscopy, light microscopy and immunostaining for cells and tissues including:

* Confocal microscopy
* Brightfield and fluorescence microscopy
* Immunoperoxidase and immunofluorescence staining

1. First time users schedule an initial meeting of the P.I., future users and core staff. During this meeting, the project and experimental details will be discussed. Core staff could suggest which microscopy modality works best for the project as well as consultation on strategies to accomplish the imaging task. Users have the option of choosing service work or independent use.

2. Schedule a service work session or a customized microscope training session with one of the core staff members.

Institutions:
  • Beth Israel Deaconess Medical Center
Personnel/Contact Information:

Director: Hagen, Susan J., Ph.D.
Phone: (617) 667-5308
Email

Member: Zheng, Yi, Ph.D.
Role: Confocal Specialist
Phone: (617) 667-1880
Email

Member: Ang, Lay-Hong, Ph.D.
Role: Confocal Supervisor
Phone: (617) 667-5373
Email

Facilities and Equipment:

Location of Core: 330 Brookline Avenue, Dana Building, Room 814, Boston, MA 02215

Major Equipment:

  • AxioVision
  • LSM 510 Software
  • Volocity
  • Zeiss Axio Imager M1 epifluorescence and brightfield microscope
  • Zeiss LSM 510 Inverted Live-Cell Confocal System
  • Zeiss LSM 510 Upright Confocal System
Services:
  • Image processing

    Includes both visualization and quantification.

  • Immunostaining (frozen and paraffin embedded tissues)

    Includes both immunofluorescence and immunoperoxidase staining.


Departmental Web Link(s):

Last updated: 2015-11-19T14:16:14.187-06:00