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Multi-Gene Transcriptional Profiling Core (BIDMC)

Director: Jaminet, Shou-Ching, Ph.D.

Location: 99 Brookline Avenue, Boston, MA 02215

Core Summary:

Multi-Gene Transcriptional Profiling (MGTP) is a real-time PCR based technique, developed in our lab, for quantifying mRNA copy numbers per cell with high reproducibility and accuracy.

Primary Goals of the MGTP Core:

* To make MGTP services available to investigators, and assist them in advancing their research and obtaining grants;
* To assist studies of molecular signaling mechanisms and pathways, discovery of biomarkers, and development of clinical assays; and
* To share the resources of our primer library, which currently includes about 2400 pre-validated human, and mouse genes.

The MGTP Core makes these unique and powerful capabilities available to biomedical researchers. Thousands of pre-validated primers are available for use, and others can be designed on demand.

Institutions:
  • Beth Israel Deaconess Medical Center
  • Harvard Medical School
Personnel/Contact Information:

Director: Jaminet, Shou-Ching, Ph.D.
Phone: (617) 667-8156

Member: Li, Dan
Role: Technician
Phone: 617-667-0155
Email

Facilities and Equipment:

Location of Core: 99 Brookline Avenue, Boston, MA 02215

Major Equipment:

  • ABI 7500 Real-Time PCR System
Services:
  • Multi-Gene transcriptional profiling (MGTP) service

    "Multi-Gene Transcriptional Profiling (MGTP) is a real-time PCR based technique, developed in our lab, for quantifying mRNA copy numbers per cell with high reproducibility and accuracy. It:

    * provides a quantitative view of systemic gene expression, and molecular fingerprinting of different cell types;
    * enables rapid identification of biomarkers for clinical applications;
    * facilitates exploration of interactions in the gene-protein network; and
    * all genes in our library of pre-validated primers are available for measurement."

  • Preparation of cDNA

    cDNA preparation from sample(s) for Multi-Gene Transcriptional Profiling (MGTP) analysis.

  • Primer design and validation

    "Primer design determines the specificity and accuracy of gene expression measurement in quantitative PCR (qPCR). All primers used in MGTP are designed within gene-specific regions through rigorous BLASTing and are validated to achieve 99.5% - 99.9% amplification efficiencies."

  • Primer distribution

    "Customers can purchase primers directly from us. For each purchase, we provide a 500 ul primer solution containing a mix of forward and reverse primers for the gene and the corresponding sequences. A current list of more than 2000 pre-validated primers can be found at the MGTP core web link."


Departmental Web Link(s):

Last updated: 2011-08-01T14:03:30.335-05:00