Longwood Small Animal Imaging Core Facility (BIDMC)
Director: Alsop, David, Ph.D.
Location: 330 Brookline Avenue, ESB-015, Boston, Massachusetts 02215
Our goal is to provide state-of-the-art small animal imaging services to researchers in the Longwood Medical Area of Harvard Medical School. These services include multi-modality imaging, advanced data analysis, image fusion resources, and a satellite animal facility for longitudinal studies. A detailed description of our instruments and services are provided on our website, and we welcome questions and comments.
Director: Alsop, David, Ph.D.
Role: Research Laboratory Manager
Phone: (617) 667-2508
Role: Research Assistant
|Facilities and Equipment:|
Location of Core: 330 Brookline Avenue, ESB-015, Boston, Massachusetts 02215
- Aspect M2 Compact High-Performance MRI System
- Bioscan NanoSPECT/CT
- CRI Maestro Multi-Spectral Fluorescence imaging system
- Cyclone Phosphor Imaging System
- Leaded Hot Cell
- Leaded Incubators
- Leica Cryostat
- Satellite Animal Housing Facility
- Secure Online iPACS System for Data Organization and Sharing (inviCRO)
- Waters HPLC
- Xenogen IVIS 50 bioluminescence imaging system
- Animal Blood Lab testing
Blood testing is a valuable tool that can reveal a wealth of data to investigators. Testing results measured against reference standards can show abnormalities, presence of disease, health of organs, and trends in experimental groups. The Longwood SAIF Blood Lab gives investigators access to a wide range of experimental tools, including:
* Vitros: Blood Chemistry and Electrolytes
* TruPoint: Blood Gases
* Hemavet: Complete Blood Count (CBC)
- Animal Handling Techniques: Injections (IV, IP), Oral Gavage, etc.
Longwood SAIF technicians are fully trained in the most common Animal Handling Techniques.
- Autoradiography and H&E Coregistration
The LSAIF provides state of the art autoradiography imaging to meet the unique needs of researchers. The LSAIF contains a Cyclone Plus Phosphor Imager that provides the quantitative images you need for all commonly used radioisotopes.
- Custom Contrast Agents
The LSAIF custom radiochemistry service can perform custom synthesis of novel F-18 labeled PET tracers such as F-18 16a-Fluoroestradiol (FES) and F-18 16b-Fluoro-5a-DiHydrotestosterone (FDHT) for small animal studies. Other F-18 custom synthesis agents are available upon request.
- In Vivo Bioluminescence Imaging
Bioluminescence imaging is a high-sensitivity, low-noise, noninvasive technique used for visualizing, tracking, and monitoring specific cellular and genetic activity in a living subject. A highly sensitive camera placed in a light-tight imaging chamber passively detects light created by luciferase. A typical image takes 1 to 5 minutes to acquire.
- In Vivo Fluorescence Imaging
Fluorescence light imaging is rapid, painless, and harmless to the subject. The typical experiment involves shining excitation light of the desired wavelength on the subject at about the same fluence rate as that of a brightly lit room. The emission light reflected from the subject is then filtered and imaged with a camera.
- In Vivo Magnetic Resonance Imaging (MRI)
Magnetic resonance imaging (MRI) is a medical imaging technique commonly used in radiology to noninvasively visualize detailed internal structures.
- In Vivo Positron Emission Tomography (PET) Imaging
Proton-rich nuclei emit positrons, antimatter, which are equal in mass and opposite in charge to an electron. When PET isotopes are used in combination with a computed tomographic (CT) scanner, investigators can quantify the exact amount of radioactivity in any organ or tissue of interest in a living animal.
- In Vivo Single-Photon Emission Computed Tomography (SPECT) Imaging
Quantify distribution of radioactive compound in living subjects to create a 3-D image. To create a 3-D image, the SPECT collimator/detector sandwich is rotated in a circular fashion around the animal; data are acquired at each angle of rotation.
- Longitudinal Animal Studies
Individual animals may be repeatedly imaged over time, enabling tracking of biological changes such as tumor growth/metabolism, spread of infection, migration of cells, and more. By imaging the same animals at all timepoints, results are more consistent; cost and usage of animals is minimized. Longitudinal studies are made easy at the Longwood SAIF with the availability of convenient on-site satellite animal housing services.
- Multimodality Coregistration with VivoQuant (inviCRO) and Interchangeable Animal Bed System
VivoQuant™ combines fundamental viewing functionality with powerful tools for fine-tuning images, isolating and analyzing regions of interest, and more. With the interchangeable Animal Bed System, coregistration is simple across PET, CT, SPECT, and MRI modalities.
- Quantitative Image Analysis: Standard Uptake Value (SUV), 3D Volume Measurements
The LSAIF employs the powerful viewing features of the VivoQuant™ multi-modality post-processing suite for SPECT, CT, PET, MR, and optical imaging data. The LSAIF also has access to an online PACS system (iPACS) that allows us to easily share dicom data and files with clients.
- Whole-body and Tissue Cryosectioning
Frozen specimens may be prepared for histological and immunological staining. Both dissected tissues and whole body axial slices can be prepared. Whole-body slices may be coregistered with imaging data, thus verifying and validating the imaging results.
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