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Harvard Digestive Diseases Center Imaging Core

Director: Massol, Ramiro, Ph.D.; Hagen, Susan J., Ph.D.; Kirchhausen, Thomas, Ph.D.

Core Summary:

Provides resources in immunofluorecence, electron microscopy, confocal, and deconvolution microscopy of living and fixed cells and tissues, and offers expertise in imaging polarized cells in monolayer culture

There are 3 locations for Core B:
* Immune Disease Institute (IDI)
* Children's Hospital Boston (CHB)
* Beth Israel Deaconess Medical Center (BIDMC)

Institutions:
  • Boston Children's Hospital
Personnel/Contact Information:

Director: Massol, Ramiro, Ph.D.
Phone: (617) 278-3210
Email

Director: Hagen, Susan J., Ph.D.
Phone: (617) 667-5308
Email

Director: Kirchhausen, Thomas, Ph.D.
Phone: (617) 278-3140
Email

Member: Wagner, Jessica
Role: Research Technologist
Email

Facilities and Equipment:

Major Equipment:

  • JEOL 1200EX electron microscope
  • Nikon E800 microscope
  • Nikon TE2000 inverted microscope
  • Reichert Ultracut E ultramicrotome
  • Zeiss Axiophot photomicroscope
  • Zeiss Axiovert 200M
  • Zeiss Axiovert 200M spinning disc confocal microscope
  • Zeiss Axiovert 200M TIRF
  • Zeiss Axiovision upright microscope
  • Zeiss LSM-510 upright confocal microscope
Services:
  • Imaging instrumentation access

    The Imaging Core makes available to the research community modern motorized microscopes capable of multiple modes of imaging of both live and fixed biological samples. In particular, our optical microscopes can perform phase and DIC imaging, widefield fluorescence, ratio imaging, laser scanning and spinning-disk confocal microscopy, dual-color total internal reflection microscopy, 5-D fluorescence imaging, FRAP, photoconversion and FRET imaging.


Departmental Web Link(s):

Last updated: 2011-07-21T09:12:46.491-05:00