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X-ray Crystallography Core (BIDMC)

Location: Beth Israel Deaconess Medical Center, X-ray Crystallography Core, Research North 325A, 99 Brookline Ave., Boston, MA 02215

Core Summary:

The X-ray Crystallography Facility located at 99 Brookline Ave. (Research North) was recently established as a core to serve the needs of investigators in the BIDMC and Harvard Medical School community interested in using structural biology as a tool in their research.

Institutions:
  • Beth Israel Deaconess Medical Center
Personnel/Contact Information:

Member: Birrane, Gabriel, PhD
Role: Instructor in Medicine
Phone: (617) 667-0025
Email

Member: General Inquiries
Email

Facilities and Equipment:

Location of Core: Beth Israel Deaconess Medical Center, X-ray Crystallography Core, Research North 325A, 99 Brookline Ave., Boston, MA 02215

Major Equipment:

  • CCP4 suite
  • DNASTAR Lasergene
  • HKL2000
  • Olympus microscope
  • Osmic confocal optics
  • R-AXIS IV image plate detector
  • Rigaku RUH3R rotating anode X-ray generator
  • Rigaku X-stream low temperature device
  • StructureStudio
  • Thermo Fisher 4ºC - 25ºC incubator
Services:
  • Access to x-ray crystallography using synchrotron radiation

    The core offers further analysis and data collection by transporting samples to the Brookhaven National Laboratory for synchrotron radiation.

  • X-ray crystallographic data analysis and processing service

    Uses a Linux workstation.

  • X-ray crystallography advising and consultation service

    X-ray crystallography support and advising services include:
    - Phasing strategy advising service (includes advice on molecular replacement and multiwavelength methods)
    - Guidance on expression and purification of macromolecules for X-ray crystal structure analysis
    - Optimization of parameters for successful crystallization
    - Optimization of cryogenic conditions

  • X-ray crystallography reference database access service

    Collection of X-ray data at ambient temperature and 100K.

  • X-ray crystallography service


Departmental Web Link(s):

Last updated: 2011-04-28T13:57:40.908-05:00