X-ray Crystallography Core (BIDMC)
Location: Beth Israel Deaconess Medical Center, X-ray Crystallography Core, Research North 325A, 99 Brookline Ave., Boston, MA 02215
The X-ray Crystallography Facility located at 99 Brookline Ave. (Research North) was recently established as a core to serve the needs of investigators in the BIDMC and Harvard Medical School community interested in using structural biology as a tool in their research.
Birrane, Gabriel, PhD
Role: Instructor in Medicine
Phone: (617) 667-0025
|Facilities and Equipment:|
Location of Core: Beth Israel Deaconess Medical Center, X-ray Crystallography Core, Research North 325A, 99 Brookline Ave., Boston, MA 02215
- CCP4 suite
- DNASTAR Lasergene
- Olympus microscope
- Osmic confocal optics
- R-AXIS IV image plate detector
- Rigaku RUH3R rotating anode X-ray generator
- Rigaku X-stream low temperature device
- Thermo Fisher 4ºC - 25ºC incubator
- Access to x-ray crystallography using synchrotron radiation
The core offers further analysis and data collection by transporting samples to the Brookhaven National Laboratory for synchrotron radiation.
- X-ray crystallographic data analysis and processing service
Uses a Linux workstation.
- X-ray crystallography advising and consultation service
X-ray crystallography support and advising services include:
- Phasing strategy advising service (includes advice on molecular replacement and multiwavelength methods)
- Guidance on expression and purification of macromolecules for X-ray crystal structure analysis
- Optimization of parameters for successful crystallization
- Optimization of cryogenic conditions
- X-ray crystallography reference database access service
Collection of X-ray data at ambient temperature and 100K.
- X-ray crystallography service
|Departmental Web Link(s):|