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Mass Spectrometry Core (BIDMC)

Location: Mass Spectrometry Core, Beth Israel Deaconess Medical Center, CLS-425, Boston, MA 02115

Core Summary:

The mass spectrometry, proteomics, metabolomics and lipidomics facility at Beth Israel Deaconess Medical Center was established in 2004 led by Dr. John Asara and continues to grow rapidly. We provide excellent proteomics and metabolomics services with low prices and fast turnaround times. We have cutting edge instruments operated at optimal sensitivity and work and publish with world class researchers. We also maintain an active internal cancer proteomics and metabolomics research program with collaborative efforts. Our expertise is in identifying and quantifying protein modifications and dynamic protein-protein interactions in addition to polar metabolomics profiling, flux analysis and lipidomics profiling. We accept collaborations from anywhere in the world and look forward to helping you and progressing your research to the next level.

Institutions:
  • Beth Israel Deaconess Medical Center
Personnel/Contact Information:

PI: Asara, John, Ph.D.
Role: Assistant Professor of Medicine
Phone: (617) 667-6182, (617) 735-2651
Email

Member: Breitkopf, Susanne, Ph.D.
Role: Research Fellow in Medicine (EXT)
Phone: (617) 735-2653
Email

Member: Yuan, Min, B.S.
Phone: (617) 735-2652
Email

Facilities and Equipment:

Location of Core: Mass Spectrometry Core, Beth Israel Deaconess Medical Center, CLS-425, Boston, MA 02115

Major Equipment:

  • AB SCIEX QTRAP 5500 mass spectrometer
  • AB SCIEX QTRAP 5500 mass spectrometer
  • Michrom Bioresources Paradigm 2D-HPLC
  • Proxeon EASY-nLC
  • Shimadzu Prominence UFLC
  • Thermo Proxeon nLC II
  • Thermo Scientific Elite Orbitrap
  • Thermo Scientific LTQ Orbitrap XL-ETD
  • Thermo Scientific Q Exactive Plus
Services:
  • 13C and 15N metabolic flux analysis

  • Mapping of post-translational modifications by LC/MS/MS

    Coomassie-stained gel bands only, purified proteins (complexes) (phosphorylation, ubiquitinylation, acetylation, etc.)

  • Metabolomics profiling service

    Includes profiling of "endogenous water soluble metabolites from any cell or tissue source."

  • Protein (complex) identification by LC/MS/MS

    Coomassie & silver-stained gel bands, solution samples after clean-up.

  • Relative quantification of peptides by LC/MS/MS

  • SILAC, iTRAQ, and Isotope-free ratio quantification service

  • Untargeted Lipidomics


Departmental Web Link(s):

Last updated: 2015-01-15T13:18:46.463-06:00