A searchable database of core laboratory facilities. Core Facilities

NextGen Sequencing Core (MGH)

Director: Sadreyev, Ruslan, Ph.D.

Location: Simches Research Center, 185 Cambridge St., Boston, MA 02114

Core Summary:

The NextGen Core is a collaboration between the Department of Molecular Biology, the Center for Human Genetics Research, the Center for Computational Biology, and the Executive Committee on Research (ECOR).

Currently, the Core operates using a single Ilumina HiSeq instrument, accompanied by Illumina's cBot for cluster generation. This upgrade from our Genome Analyzer II doubled our capacity and greatly increased the data amount, quality, and stability over extra-long reads.

The Core is located in the state-of-the-art Richard Simches Research Center on Cambridge St. as part of the MGH main campus in Boston. The many multi-investigator groups in the building - including those that study human genetics, stem cells, genomics, and more - make it the perfect location for the Core to service the researchers in those groups. The majority of customers come from MGH, but we also service customers at other academic medical centers and industry.

Institutions:
  • Massachusetts General Hospital
Personnel/Contact Information:

Director: Sadreyev, Ruslan, Ph.D.
Phone: (617) 643-5697
Email

Member: General Inquiries
Email

Facilities and Equipment:

Location of Core: Simches Research Center, 185 Cambridge St., Boston, MA 02114

Major Equipment:

  • Agilent Bioanalyzer
  • Covaris S2
  • Illumina HiSeq 2000
Services:
  • Data Analysis

    "Illumina's HiSeq software automatically makes base calls and generates FASTQ sequence and quality files, which are delivered to you. As a separate service, the core's experienced bioinformatics team can perform custom alignment and downstream analysis relevant to your project. For this service contact bioinfo@molbio.mgh.harvard.edu"

  • Library Validation

    "We strongly recommend that all libraries are validated and quantitated using Bioanalyzer, qPCR, or similar methods before submission. In addition, the core staff validates all submitted libraries before sequencing to ensure maximum quality and cluster yield. This service is included in the sequencing cost. Please click the "library validation" box in Galaxy when submitting your samples if you are submitting barcoded subsamples and need us to pool them for you."

  • NextGen sequencing

    "Up to 7 samples (plus a control) may be run on a single flow cell, or multiple samples may be multiplexed in one lane if obtaining the absolute highest coverage is not necessary or if previous runs have yielded excessive coverage (this may reduce costs). When deciding, keep in mind that our HiSeq is currently putting out approximately 150 million reads. Our core is actively engaged with Illumina scientists and other NextGen sequencing leaders to ensure we are up-to-date with reagents, software, and parts, and quality is constantly monitored throughout the run.

    We currently offer:

    • 50 cycle single read or paired end reads
    • 100 cycle single read or paired end reads
    • Multiplexing for any read type"


Departmental Web Link(s):

Last updated: 2015-11-19T12:48:43.081-06:00