A searchable database of core laboratory facilities. Core Facilities

Cell, Tissue and Organ Resource Core (MGH)

Director: Uygun, Korkut, Ph.D.

Core Summary:

The mission of the Cell Resource Core (CRC) is to provide high quality primary cells and tissues to researchers reliably and affordably. Leveraging a new MGH technology that significantly increases the viable yield of high quality hepatocytes from human and animal donor livers, we are initially offering hepatocytes to the research community.

  • Massachusetts General Hospital
Personnel/Contact Information:

Director: Uygun, Korkut, Ph.D.

Member: Geerts, Sharon
Role: Accounts Manager

Member: Ozer, Sinan
Role: Operations Manager

Member: Chu, Chris
Role: Isolations Technician

  • Cryopreserved hepatocytes

    Cryopreserved hepatocytes are available on demand. These cells are the same fresh rat hepatocytes preserved with standard cryopreservation protocols. Please note that post-thaw viability and functionality of cryopreserved hepatocytes are notably below those of fresh cells.

  • Custom hepatocyte isolations

    Custom hepatocyte isolations (species/strain) will be considered on a client-to-client basis. Please inquire.

  • Decellularized whole rat livers

    Interested in tissue engineering but have limited access to animal/surgical resources? We now provide decellularized whole rat livers for regenerative medicine studies employing native liver ECM. Full details of these scaffolds can be found in a recent publication:

  • Fresh hepatocytes in suspension

    Our primary rat hepatocytes are isolated from female Lewis rats, with >90% viability at time of isolation and >99% purity. Isolations are done on a weekly basis and can be tracked via our google calendar. Cells are provided in vials suspended in C+H media on ice, and typically remain highly viable up to 6 hours as long as kept cold.

    Fatty hepatocytes are also available from obese diabetic male Zucker rats. Additional strains are available upon request.

  • Plated hepatocytes

    Plated hepatocytes can be provided in various formats (6 well, collagen sandwich etc). Typical configuration is 6 well plate in collagen sandwich and C+H media, which takes ~4 days to be ready after the day of isolation. Please inquire.

Departmental Web Link(s):

Last updated: 2015-12-14T13:12:12.121-06:00