Ragon Institute Imaging Core, Flow Cytometry (MGH)
Director: Diefenbach, Thomas J., Ph. D.; Waring, Michael T.
Location: 400 Technology Square, 9th floor, Cambridge, MA, 02139
The mission of the Ragon Institute Imaging Core is to bring the latest imaging modalities to bear on fundamental molecular and cell biological questions pertaining to infectious diseases. As an MGH core, it also serves the greater MGH community in all aspects of microscopy, flow cytometry and cell sorting. The Flow Cytometry division of the Ragon Institute Imaging Core is located on the 9th floor of 400 Technology Square in Cambridge. The core offers training, assistance and access to flow cytometers and other instrumentation. Consultation is provided on experimental design. Our high speed cell sorter is situated in a BL2+ facility, permitting sorting of fixed or live samples with up to BL2+ level of biosafety.
Since the Ragon Institute is also a collaboration between MGH, Harvard, and MIT, so too does the facility welcome members of the MIT and Harvard communities.
Director: Diefenbach, Thomas J., Ph. D.
Phone: (857) 268-7046
Director: Waring, Michael T.
Phone: (857) 268-7069
|Facilities and Equipment:|
Location of Core: 400 Technology Square, 9th floor, Cambridge, MA, 02139
- 3 Laser LSRII (BD Biosciences)
- 4 Laser, 13 color LSR II (BD Biosciences)
- 4 Laser, 18 color LSR II (BD Biosciences)
- Analysis Stations
- AutoMACS cell separator (Miltenyi Biotech)
- Bioplex 3D multiplex analyzer (Bio-Rad)
- Sony SH800 Cell Sorter (Sony Biotech)
- SORP FACSAria II (BD Biosciences)
- Two 5 Laser LSR Fortessa (BD Biosciences)
- Assisted Flow Cytometry
Users may have assistance in the use of the flow cytometers.
- Flow Cytometric Cell Sorting
Users can book time with core personnel to have their samples run on a cell sorter to purify target populations of interest. The FACSAria IIu sorter is housed in a biosafety cabinet to allow for safe processing of primary human samples or infectious samples. Up to 4 populations can be selected at a time.
- Flow Cytometry Instrumentation Access
Trained users are permitted to sign up for and use the flow cytometers for analysis at their convenience.
- Flow Cytometry Training
The core offers a training regimen to prepare researchers to become independent operators of our instrumentation. The full syllabus is outlined on the “Training” page found via the link below, and includes an “Introduction to Flow Cytometry” seminar and hands-on instruction in instrument operation and optimization.
|Departmental Web Link(s):|