Ragon Institute Imaging Core, Microscopy (MGH)
Director: Alter, Galit, Ph.D.
Location: 400 Technology Square, Rooms 810A and 812, Cambridge, MA, 02129
The mission of Ragon Institute Imaging Core, Microscopy Division is to bring the latest imaging modalities and technology to bear on fundamental molecular and cell biological questions pertaining to infectious diseases. As an MGH Core Facility, the Ragon Institute Imaging Core also serves the MGH greater MGH community. Since the Ragon Institute is also a collaboration between MGH, Harvard, and MIT, so too does the facility welcome members of the MIT and Harvard communities as we are now located in Cambridge at MIT.
The facility encompasses five imaging systems, including a Zeiss LSM510 laser scanning confocal microscope and a fully automated Zeiss Axio Observer microscope, both housed in BL2+ compliant facilities. These imaging systems are therefore fully equipped for both fixed and live cell imaging. In addition, the facility has two slide scanning systems (MIRAX MIDI and TissueFAXS) for high speed automated imaging and cellular screening of tissue sections and cultured cells on glass slides. In the Flow Cytometry section of the Imaging Core, we also have an ImageStream X Mark II by Amnis.
The core also serves as a resource for addressing a variety of imaging needs including consultation on a topics related to imaging applications, experimental design, and sample preparation.
Director: Alter, Galit, Ph.D.
Phone: (857) 268-7003
Diefenbach, Thomas J., Ph. D.
Role: Associate Director, Microscopy
Phone: (857) 268-7046
|Facilities and Equipment:|
Location of Core: 400 Technology Square, Rooms 810A and 812, Cambridge, MA, 02129
- High-end image analysis HP PC workstation
- ImageStreamX Mark II by Amnis for imaging up to 5000 cells/second with up to 12 color channels
- Imaris Image Analysis Software Suite with Imaris Batch (Bitplane)
- Metamorph Image Analysis Software (Molecular Devices)
- TissueFAX Slide Scanner by TissueGnostics (brightfield & fluorescence)
- TissueQuest (fluorescence) and Histoquest (brightfield) Image Analysis Software (Tissue Gnostics)
- Zeiss Axio Observer A1 inverted fluorescence microscope
- Zeiss Axio Observer Z1 automated/high-speed inverted microscope
- Zeiss LSM510 laser scanning confocal inverted microscope
- Zeiss MIRAX MIDI Slide Scanner (brightfield & fluorescence)/
- Assisted Microscopy
Users may have assistance in the use of the imaging systems.
- Microscopy Instrumentation Access
The confocal and RPI imaging systems are housed in BL2+ compliant facilities and equipped for live cell imaging in addition to imaging of fixed preparations. The imaging systems cover a wide range of imaging applications including automated multi-well plate imaging, whole-slide fluorescence and brightfield scanning (for large area image reconstruction and analysis), high-speed multi-color fluorescence imaging, long term time-lapse imaging, and confocal and wide-field live cell or tissue time-lapse imaging (multiple positions in either 2D or 3D). Cell-specific applications include FRET, FRAP, intracellular particle tracking, 3D reconstruction, internalization studies, cell motility and tracking, cell population screening in tissue sections and cell culture similar to FAXS, and up to six color fluorescence imaging on the confocal microscope.
- Microscopy Training Courses
A Microscopy Fundamentals training class is held on a regular basis or based on demand. Each class is 1.5 hours long and provides a general introduction to the principles and applications of microscopy, including fluorescence microscopy and confocal imaging. Additional classes on specific applications are available on demand.
- Training in Microscopy/Imaging
Users are given full training for independent use of the imaging systems.
|Departmental Web Link(s):|