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Genome Engineering Production Group (HMS)

Director: Marsischky, Gerald, Ph.D.; Mohr, Stephanie, Ph.D.

Location: Department of Genetics, 77 Avenue Louis Pasteur, New Research Building 336, Boston, MA 02115

Core Summary:

The GEPG provides researchers with easy, low cost access to reagents for genome engineering. We can custom design and build TALENs, CRISPR sgRNAs, and donor constructs for knock-out or knock-in approaches.

Institutions:
  • Harvard Medical School
Personnel/Contact Information:

Director: Marsischky, Gerald, Ph.D.
Email

Director: Mohr, Stephanie, Ph.D.
Phone: (617) 432-5626
Email

Facilities and Equipment:

Location of Core: Department of Genetics, 77 Avenue Louis Pasteur, New Research Building 336, Boston, MA 02115

Major Equipment:

  • QIAxcel Advanced System capillary electrophoresis device for RNA and DNA analysis
Services:
  • Custom CRISPR design and construction

    Design of CRISPR short guide RNAs (sgRNAs) using up-to-date rules for effectiveness and reduced off-targeting; custom construction of CRISPR sgRNA constructs in a variety of vectors.

  • Custom donor construct construction

    Custom construction of donor constructs for knock-in approaches.

  • Custom TALEN construction

    Custom construction of TAL endonuclease (TALEN) pairs and other TAL fusions.

  • Project consultation

    Consultation on small or large-scale genome engineering projects in mammalian or other systems.


Departmental Web Link(s):

Last updated: 2014-05-09T12:39:27.268-05:00