A searchable database of core laboratory facilities. Core Facilities

Molecular Medicine Core (BIDMC)

Director: Balk, Steven, M.D., Ph.D.; Petkova, Victoria, Ph.D. (Co-Director)

Location: 330 Brookline Avenue, Beth Israel Deaconess Medical Center, SL232-The Slosberg Building, Boston, MA 02215

Core Summary:

The core is equipped with the following instruments: Illumina MiSeq Next Generation Sequencer, Arcturus XT Laser Capture Microdissection System, Agilent 4200 TapeStation Bioanalyzer, Covaris E220 Ultra-Sonicator, ABI 7000 and 7500 Fast Real-Time qPCR systems, Li-Cor Odyssey System Infrared Imager.

On an hourly fee basis we provide: training on equipment usage, consultations, core setup, data analysis, troubleshooting, primer design, LCM usage. On per sample or per run fee basis we provide: qRT-PCR runs, quality and quantity DNA and RNA analysis, Covaris ultrasonicator sample shearing, LiCor scanning.

For details contact Dr. Petkova.

  • Beth Israel Deaconess Medical Center
Personnel/Contact Information:

Director: Balk, Steven, M.D., Ph.D.
Phone: (617) 667-3918

Director: Petkova, Victoria, Ph.D. (Co-Director)
Phone: (617) 667-0603

Facilities and Equipment:

Location of Core: 330 Brookline Avenue, Beth Israel Deaconess Medical Center, SL232-The Slosberg Building, Boston, MA 02215

Major Equipment:

  • ABI 7000 Real-Time PCR System
  • ABI 7500 Fast Real-Time qPCR system
  • Agilent 2200 TapeStation Bioanalyzer
  • Arcturus XT Laser Capture Microdissection System
  • Covaris E220 Ultra-Sonicator
  • Illumina MiSeq Next Generation Sequencer
  • Li-Cor Odyssey System Infrared Imager
  • ArcturusXT LCM usage

    After being trained on the proper usage of the LCM microscope, you will be free to reserve time on the LCM through an on-line calendar and work with the equipment. Come over and enjoy this powerful, state-of-the art instrument.

  • Consultations on Real-Time PCR Technology and Experimental design

  • Core setup of Real-Time PCR runs Probe/primer design

  • Covaris Ultrasonicator sample shearing

  • Data analysis troubleshooting

  • Illumina MiSeq Next Generation Sequencer

    You bring your libraries for sequencing, we run them and provide you with the .fastq for further analysis.

  • Li-Cor Odyssey Infrared Imaging

  • qRT-PCR runs

    Real-Time PCR will always remain the technology you will need for the final touch of your gene expression studies. Our core is equipped with all an investigator needs for primer design, experimental run and data processing. If you have been trained to operate a Real-Time PCR machine you can perform the experiment yourselves. 
Or, we can do it for you. You can purchase from us all the necessary reagents, endogenous controls and consumables. Or, you can use your own.

  • Quality and Quantity DNA/RNA sample analysis on Agilent 4200 Tape Station

  • Training on equipment usage

Departmental Web Link(s):

Last updated: 2017-03-13T12:44:25.239-05:00