Neurobiology Imaging Facility (HMS)
Location: Harvard Medical School, Goldenson Building 242, Boston, MA 02115
The Neurobiology Imaging Facility is the Neurobiology Department core facility for light microscopy.
Our mission is to advance research by providing services in optical imaging, introducing new optical imaging equipment, providing learning opportunities to researchers in the Longwood area and increasing the general imaging expertise of neuroscientists.
Role: Core Facility Manager, Senior Imaging Specialist
Phone: (617) 432-1683
|Facilities and Equipment:|
Location of Core: Harvard Medical School, Goldenson Building 242, Boston, MA 02115
- BX63 Motorized Fluorescent Microscope
- FV1000 Laser Scanning Confocal Microscope
- FV1200 Laser Scanning Confocal Microscope with Tokai Hit Environ Chamber and laser based auto-focus
- MMI CellCut Laser Dissection Microscope
- MVX10 Macroview Dissecting Microscope
- Offline Workstations
- Olympus R1 Cell Counter
- VivaView FL Incubator Fluorescence Microscope
- VS120 Slide Scanner
- VS120 Slide Scanner
- Zeiss COMBI System-Microdissection and optical tweezers
- Array Tomography (AT) Core
The Array Tomography Core (AT Core) provides high resolution 3D reconstruction of tissue below the diffraction limit. We circumvent this limitation through physical serial sectioning of tissue below the diffraction limit. That is, we embed tissue (usually murine brain) in LR White resin then use an ultramicrotome to serially section the tissue at ~70 nm. These tissue arrays are then probed using various antibodies specific to desired antigens, usually pre- and post-synaptic markers, via standard immunohistochemistry techniques. The stained arrays are then imaged using a widefield epifluorescent scope and the resulting images are aligned and the final 3D volume rendered in various software (Amira, ImageJ, Metamorph).
- Imaging Equipment Access Service
Instrumentation access provided to authorized researchers.
"The Neurobiology Imaging Core Facility will provide training sessions for free. You will be expected to safely turn on and run the equipment and know the shutdown routine before you can use the equipment autonomously.
Offline workstations loaded with Metamorph, Imaris, ImageJ and CellSens are provided for post-acquisition analysis.
Long term data storage is not provided. Users must use their own collaboration folders on the HMS server or save their data on a personal device. Data over 1 month old will be deleted.
PUBLICATION ACKNOWLEDGEMENT: If research supported by the Neurobiology Imaging Facility results in publication, please acknowledge this support by including the following statement in your publication(s):
We thank the Neurobiology Department and the Neurobiology Imaging Facility for consultation and instrument availability that supported this work. This facility is supported in part by the Neural Imaging Center as part of an NINDS P30 Core Center grant #NS072030."
|Departmental Web Link(s):|