A searchable database of core laboratory facilities. Core Facilities

Small Molecule Mass Spectrometry Facility (FAS)

Director: Trauger, Sunia

Location: Northwest Laboratory building, B2, 52 Oxford Street, Cambridge, MA, 02138

Core Summary:

The Small Molecule Mass Spectrometry Facility at the FAS Center for Systems Biology offers support for the analysis of a wide variety of analytes using mass spectrometry based techniques. We are located in the second basement (B2) level of the Northwest Laboratory building at 52 Oxford Street, Cambridge, MA 02138. We provide services for molecular formula confirmation (accurate mass measurement), structural elucidation (MS/MS) and quantitation of small molecules. In addition, we can assist you in the mass analysis of a wide variety of non-proteomics samples including metabolites, medium sized proteins and oligonucleotides. Please contact us and tell us about your samples prior to submission or use of our Open Access laboratory.

Institutions:
  • FAS Center for Systems Biology
Personnel/Contact Information:

Director: Trauger, Sunia
Phone: (617) 384-9585
Email

Member: General Inquiries
Phone: (617) 384-9485
Email

Facilities and Equipment:

Location of Core: Northwest Laboratory building, B2, 52 Oxford Street, Cambridge, MA, 02138

Major Equipment:

  • Agilent 1100 HPLC with UV Detector
  • Agilent 6120 Quadrupole LC/MS with Multimode Source
  • Agilent 6210 Time-of-Flight LC/MS
  • Agilent 6460 Triple Quadrupole Mass Spectrometer with Agilent 1290 uHPLC
  • Bruker DataAnalysis and Compas
  • Bruker Maxis Impact q-TOF Mass Spectrometer
  • Chemstation
  • MassHunter
  • MassLynx
  • ThermoXCalibur
  • TSQ Quantum Access MAX Triple Stage Quadrupole Mass Spectrometer
  • Waters MALDI-TOF
  • Waters Quattro micro GC/MS/MS
Services:
  • High Resolution LC-MS

    Samples will be analyzed using high resolution mass spectrometry. Interpreted data report will be provided to the user ~24 hours after sample submission. This service is available to all.

  • Mass Spectrometry Analysis

    Fee-for-service MS Analysis

  • Protein LC-MS Analysis

    LC-MS will be used to analyzed proteins of interest. Interpreted report providing accurate MW information (typically within 1 Da) will be provided to the investigator. This technique is ideal for studying covalent attachments to proteins of interest.

  • Quantitative Analysis of Small Molecules

    Using standard compounds, an optimum HPLC-MS/MS method will be developed for the molecule/molecules of interest. After this samples will be quantified using a standard curve based on internal or external standards on a triple quadrupole mass spectrometer.


Departmental Web Link(s):

Last updated: 2012-07-12T12:58:40.299-05:00