A searchable database of core laboratory facilities. Core Facilities

Neurodevelopmental Behavior Core (BHC)

Director: Fagiolini, Michela, Ph.D.

Location: Karp Building, Boston Children’s Hospital, Boston, MA, 02115

Core Summary:

The Neurodevelopmental Behavior Core (NBC) was designed to provide a time-efficient and cost-effective service for the comprehensive characterization of complex behaviors in mouse models of neurodevelopmental disorders, and for testing novel therapeutic drugs and interventions in mouse models of these human disorders. The NBC provides the necessary equipment, protocols and technical support for studying neurodevelopmental disorders, focusing not just on “snapshots” but changes in developmental milestones over time. The Core is equipped to perform extensive batteries of behavior tests that phenotype specific social, emotional and cognitive behaviors, as well as motor, auditory and visual function, together with measures of the general health of the animals. This behavior facility also provides a unique opportunity for training fellows, graduate and undergraduate students as well as PIs.

The NBC is available for use by BCH and non-BCH scientists.

Institutions:
  • Boston Children's Hospital
Personnel/Contact Information:

Director: Fagiolini, Michela, Ph.D.
Phone: (617) 919-2419
Email

Member: Andrews, Nick, Ph.D.
Role: Core Manager
Phone: (617) 919-3667
Email

Facilities and Equipment:

Location of Core: Karp Building, Boston Children’s Hospital, Boston, MA, 02115

Major Equipment:

  • Digigait
  • ECG
  • EEG: juvenile (tethered) and adult (telemetry)
  • Ethovision XT video tracking systems
  • Home cage activity system
  • Home cage cognition wall
  • Inframot
  • in vivo microdialysis
  • Marlau cages
  • Running wheels
  • Stereotaxic frames
  • Treadmill
  • Whole Body Plethysmography system
Services:
  • Auditory function tests

    Auditory brain stem responses
    Ultrasonic vocalisation detection

  • Circadian cycle tests

    Home cage activity

  • Cognition tests

    Operant behavior touchscreens
    Morris water maze
    Novel object recognition
    Social recognition
    Y-Maze
    Water T-maze

  • Dosing and tissue sampling for pharmacokinetics analysis

  • Emotion tests

    Elevated plus maze
    Open field
    Light/dark box
    Conditioned fear
    Social interaction
    Chronic mild stress
    Sucrose drinking
    Forced swim
    Tail suspension

  • Global Phenotyping

    SHIRPA

  • In vivo phenotyping of mice

  • Motor function tests

    Holeboard
    Rotarod
    Beam walk
    Grip-strength

  • Nociception tests

    Hotplate
    Thermal place preference
    Thermal gradient
    Dynamic weight bearing
    von Frey

  • Physiology Tests

    Whole body plethysmography
    EEG: juvenile (tethered) and adult (telemetry)

  • Rearing manipulation tests

    Dark rearing
    Sound rearing
    Isolation

  • Stereotaxic surgery

  • Visual function tests

    Optomotor
    Visual cliff
    Looming test
    Visual water task
    Eye tracking


Departmental Web Link(s):

Last updated: 2017-11-15T11:30:32.214-06:00