A searchable database of core laboratory facilities. Core Facilities

Boston Children's Hospital Viral Core

Director: He, Zhigang, Ph.D.

Location: 3 Blackfan Circle, Center For Life Science Building, Room13060, Boston, MA, 02115

Core Summary:

Viral Core’s goal is to provide scientists with access to cutting-edge viral vector technologies in an effective, high-quality and cost-efficient fashion. Viral Core also provides consultation to investigators in selecting, designing and using viral vectors.

Institutions:
  • Boston Children's Hospital
Personnel/Contact Information:

Director: He, Zhigang, Ph.D.
Phone: (617) 919-2353
Email

Member: Wang, Chen, Ph.D.
Role: Core Manager
Phone: (617) 919-2271
Email

Facilities and Equipment:

Location of Core: 3 Blackfan Circle, Center For Life Science Building, Room13060, Boston, MA, 02115

Major Equipment:

  • Beckman Coulter Avanti J-E centrifuge
  • Bio-Rad CFX Connect Quantitative PCR machine
  • Eppendorf 5810R centrifuge
  • GE AKTA Purifier FPLC
  • Sonicator
  • Thermo biosafety hood
  • Thermo HEPA incubators
Services:
  • Custom made AAV prep

    We purify AAVs using Iodixanol gradients and ultracentrifugation. We could prep AAV vectors into serotype 1, 2, 5, 6, 7, 8, 9, rh10 and DJ. For a regular AAV prep, we need at least 150ug maxi-prep plasmid DNA. Typical virus yield is between 250-400ul. Genomic titer is quantified using Q-PCR. Purified AAV is good for in vivo applications.

  • Custom made lenti prep

    Our lenti virus is pseudotyped with vsvg. We concentrate lenti virus by ultracentrifugation and quantify its genomic titer by Q-PCR.
    Please provide at least 100ug plasmid DNA for a lenti prep. Typical virus yield is between 150-200ul.

  • Pre-made AAV prep

    We provide pre-made AAVs with different serotypes for AAV-GFP, AAV-Cre and AAV-ChR2. For detailed information of in stock pre-made AAVs, please email chen.wang@childrens.harvard.edu.


Departmental Web Link(s):

Last updated: 2015-12-16T12:51:18.711-06:00