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Transgenic Mouse Core (HMS)

Director: Sharpe, Arlene H., M.D., Ph.D.

Location: New Research Building, Rm. 837, 77 Ave. Louis Pasteur, Boston, MA 02115

Core Summary:

Established in 1992, the Transgenic Mouse Core provides services for the generation of transgenic and knockout mice using state-of-the-art facilities and equipment.

Dr. Lina Du, formerly a plastic surgeon in Beijing and an expert in microvascular surgery, performs both blastocyst and pronuclear injections. She has over 20 years of experience in embryo manipulation and the generation transgenic mice.

The Transgenic Mouse Core has all of the equipment necessary for generation of transgenic mice including a Nikon Diaphot microscope equipped with Nomarski Optics and Narishige micromanipulators for microinjections, Nikon surgical microscopes for egg isolation ad transfer, a sutter needle puller and a de Fonbrune microforge. The facility maintains mice necessary for egg donors, egg recipients, and vasectomized males. This is a non-profit facility and charges are based upon anticipated mouse costs, maintenance of mice and equipment, purchase of necessary surgical supplies and chemicals, and personnel costs.

ES cell culture services have been offered for 15 years. Our staff carries out electroporation of targeting vectors into ES cells and provides investigators with DNA to identify ES cells carrying the desired recombination events. Staff then expand the appropriate ES cells for microinjection into blastocysts by the Transgenic Mouse Core.

In recent years, the core has expanded its list of services to include CRISPR injections and cryopreservation.

Institutions:
  • Harvard Medical School
Personnel/Contact Information:

Director: Sharpe, Arlene H., M.D., Ph.D.
Phone: (617) 432-6569
Email

Member: Du, Lina, M.D.
Role: Transgenic Specialist
Phone: (617) 432-6227
Email

Member: Wyner, Lauri
Role: Content Manager
Phone: (617) 432-4947
Email

Member: Peluso, Michael
Role: ES Cell Culture Technician
Phone: (617) 432-6182
Email

Member: General Inquiries
Phone: (617) 432-6182
Email

Facilities and Equipment:

Location of Core: New Research Building, Rm. 837, 77 Ave. Louis Pasteur, Boston, MA 02115

Major Equipment:

  • De Fonbrune microforge
  • Electroporator
  • Nikon Diaphot microscope equipped with Nomarski Optics and Narishige micromanipulators for microinjections
  • Nikon surgical microscopes for egg isolation and transfer
  • Sutter needle puller
Services:
  • BAC injection services

  • Breeding services

  • CRISPR Injection services

  • ES cell electroporation services

  • ES cell injection services

  • Fresh/Frozen sperm IVF

  • Inducible progenitor stem cells (IPS) injection

  • Injection of DNA constructs (BALB C, C57BL/6, FVB) into egg pronuclei for generating transgenic mice

  • Lentiviral Injection services

  • Sperm/embryo cryopreservation

  • Tail biopsy services

  • Thawing and implantation of embryos


Departmental Web Link(s):

Last updated: 2017-01-05T12:29:17.668-06:00