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CyTOF Antibody Resource and Core

Director: Cahill, Laura

Location: Brigham and Women’s Hospital, 20 Shattuck Street, Thorn Building, Room 425, Boston, MA 02115

Core Summary:

Our core specializes in conjugating purified antibodies to a 35-metal catalogue for CyTOF users. With over 429 unique markers (261 for human and 168 for mouse), any user can create their own panel from our inventory. We also have optimized protocols specific to CyTOF for cell surface, intracellular cytokine, transcription factor, and phospho-signaling stains. All protocols have upstream specific techniques optimized for single cell population preservation following cryopreservation and through the entirety of the staining procedure.

We are akin to flow cytometry, but we actually utilize mass cytometry (CyTOF).

Institutions:
  • Brigham and Women's Hospital
Personnel/Contact Information:

Director: Cahill, Laura
Phone: (323) 404-7092
Email

PI: Lederer, James Ph.D.
Role: Associate Professor
Phone: (617) 732-7399
Email

Facilities and Equipment:

Location of Core: Brigham and Women’s Hospital, 20 Shattuck Street, Thorn Building, Room 425, Boston, MA 02115

Major Equipment:

  • Cytobank
Services:
  • Conjugated Antibody Requests

    For Harvard-affiliated institutions, the price for a standard antibody vial (40 uL) is $70. For custom conjugations, the price is $230 for a vial (200 uL). For institutions not affiliated with Harvard, the respective prices are $101 and $331. Please download a request template from our website for all orders.

  • Panel Design and Staining Protocols

    Knowledge of metal cross-talk and antibody sensitivity/relative protein abundance is used to carefully design each panel. Optimized protocols specific to CyTOF include cell surface, intracellular cytokines, transcription factors, and phospho-signaling stains. If interested, please e-mail Laura Cahill at LCahill5@bwh.harvard.edu.


Departmental Web Link(s):

Last updated: 2017-06-22T13:16:28.061-05:00