Cell Culture and Microscopy Core
Director: James Rheinwald, Ph.D.
Location: 77 Avenue Louis Pasteur, HIM 660, Boston.
| Core Summary: |
The following Cell Culture and Microscopy services are currently provided by this core:
1) Primary and experimentally immortalized human skin fibroblast and keratinocyte cell lines and squamous cell carcinoma cell lines.
2) Initiation of new primary skin-derived cell lines from humans with inherited diseases or syndromes and genetically modified mice.
3) Starter samples of specialized media formulations and pre-tested serum for various cell types.
4) Training in cell culture and advice on design of experiments using primary skin cells.
5) Training and access to a fluorescence microscope and digital camera system.
| Categories and Institutions: |
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| Personnel/Contact Information: |
Director: James Rheinwald, Ph.D.
Phone: 617-525-5553
Fax: 617-525-5571
Email
Member: Patricia Barron
Role: Core Manager
Phone: 617-525-5562
Fax: 617-525-5571
Email
| Facilities and Equipment: |
Location of Core: 77 Avenue Louis Pasteur, HIM 660, Boston.
Major Equipment:
- Cell culture laboratory
- Nikon phase contrast and Nomarski optics microscope equipped for fluorescence microscopy and digital photography
| Services: |
- Consultation and custom culture services
- Cryopreservation and storage of cultures
- Genetic engineering of such cells using retroviral or lentiviral vectors
- Initiation of primary fibroblast and keratinocyte lines from biopsies of human tissue (e.g., generation of primary cell lines from individuals known to carry a specific gene mutation)
- Instruction in the use of and access to our phase contrast/fluorescence microscope, digital camera, and computer with photo-editing software
- Provision of optimized culture medium formulations for various cells types
- Training in cell culture and experimental design using human cell lines
- Well-characterized normal primary human epidermal, oral, and ectocervical keratinocyte, connective tissue fibroblast, large vessel endothelial, and pleural mesothelial cell lines and cell lines cultured from squamous cell carcinomas
| Departmental Web Link: |
http://rheinwaldlab.bwh.harvard.edu/core.html