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McLean Hospital Microscopy Core Facility

Director: Berretta, Sabina, M.D.

Location: McLean Hospital, 115 Mill St, Belmont, MA 02478, MRC, Basement, Room # 029-030

Core Summary:

The McLean Hospital Microscopy Core Facility, located in the Mailman Research Center (MRC), is a shared user facility that supports basic scientific imaging and cell sorting research efforts within the McLean Hospital research community. Users from other Institutions are also welcome, with prior approval from the Core Director. Major instrumentation offered by the Core includes a JEOL JEM1200EX Transmission Electron Microscope (TEM), a recently acquired state-of-the-art Leica SP8-TCS Laser Scanning Confocal Microscope (LSCM), and a newly acquired Bio-Rad S3 Fluorescence Activated Cell Sorter (FACS).

Institutions:
  • http://harvard.eagle-i.net/i/0000012a-2512-1b62-5617-794280000000
  • Partners HealthCare
Personnel/Contact Information:

Director: Berretta, Sabina, M.D.
Phone: 617-855-3484
Email

Member: Berciu, Cristina, Ph.D.
Role: Core Manager
Phone: 617-855-2798
Email

Member: General Inquiries
Email

Facilities and Equipment:

Location of Core: McLean Hospital, 115 Mill St, Belmont, MA 02478, MRC, Basement, Room # 029-030

Major Equipment:

  • JEOL JEM1200EX transmission electron microscope (TEM)
  • Leica Laser Scanning Confocal Microscope TCS-SP8
  • S3e Cell Sorter (FACS)
Services:
  • Confocal Microscopy Service


    • One-on-one training for using the confocal microscope

    • Consultation/collaboration on sample preparation, experimental design, image analysis

    • Image acquisition

  • Fluorescence Activated Cell Sorting (FACS) Service


    • One-on-one training for using the FACS machine

    • Consultation on sample preparation

  • Transmission Electron Microscopy Service


    • Consultation on project and sample preparation

    • Sample preparation for TEM (fixation, infiltration and embedding in resin, sectioning and staining)

    • Image acquisition and interpretation


Departmental Web Link(s):

Last updated: 2017-09-14T09:55:38.554-05:00