A searchable database of core laboratory facilities. Core Facilities

Transgenic Core Laboratory (BCH)

Director: Woolf, Clifford, M.D., Ph.D.

Core Summary:

The Transgenic Core centralizes the production of genetically altered mice for MRRC investigators in a cost-effective and efficient manner. The Core also provides expert assistance in the additional areas required for generating genetically altered mice, including transgene and targeting construct production, and transgenic mouse colony management. Core personnel are available to train investigators in all of the techniques required for the generation of genetically modified mice.

Institutions:
  • Boston Children's Hospital
Personnel/Contact Information:

Director: Woolf, Clifford, M.D., Ph.D.
Phone: (617) 919-2393
Email

Member: Bhaumik, Mantu Ph.D.
Role: Research Fellow in Neurology
Phone: (617) 919-2128
Email

Member: General Inquiries
Email

Facilities and Equipment:

Major Equipment:

  • BioCool-IV Controlled rate Freezer
  • BioRad Gene Pulsar electroporator
  • BTX ECM 2001 electroporator
  • De Fonbrune microforge
  • Eppendorf Centrifuge 5702R
  • Eppendorf Femtojet microinjector
  • Eppendorf Transferman 4r (2)
  • Eppendorf Transferman NK (2)
  • Kopf Vertical pipet puller
  • Leica M205C & MC170 HD Camera
  • MJ Research PTC-100 PCR Machine
  • Narishige EG-400 microgrinder
  • Nikon Diaphot 200 inverted microscope (4X, 20X, 10X & 20X DIC)
  • Nikon SMZ-2B dissecting microscope (2)
  • Nikon SMZ-U dissecting microscope
  • Nikon TE2000S inverted microscope for microinjection setup (4X, 20X, 20X DIC)
  • Nikon TE2000U inverted microscope for microinjection setup (4X, 20X & 40X Hoffman)
  • Nikon TMS microscope
  • PrimeTech Piezo Drill PMAS-CT150
  • REVCO Ultima CO2 Incubator
  • Sutter Instrument Flaming/Brown P87 horizontal micropipette puller
  • TECHNE Hybridiser Oven
  • Thermo Scientific -80/TSU Series
  • Thermo Scientific CO2 incubator Series 3 WJ
  • Thermo Scientific CryoPlus2
  • Warner Instruments PL1-100A Pico liter injector
Services:
  • Gene targeting service

    Can be used with either pronuclear and blastocyst injection setups.

  • Transgenics service

    Can be used with either pronuclear and blastocyst injection setups.


Departmental Web Link(s):

Last updated: 2015-12-02T20:19:05.606-06:00