Imaging Core
Director: Scott Pomeroy
Location: Children's Hospital 320 Longwood Avenue Suite 375 Boston, MA 02115
| Core Summary: |
The Imaging Core has evolved into a facility that offers investigators a broad range of imaging technologies that are both easy to use and affordable. Investigators have used the spatial and temporal resolution capabilities of the confocal system for projects ranging from intracellular localization of molecules to time-lapse studies of the movement of ligand-receptor complexes through axons. Our new digital microscope has further expanded the scope of the Imaging Core, allowing investigators to work with brightfield and darkfield formats that are critical for analysis of histological sections, as well as with phase and differential interference contrast. Moreover, this microscope adds the morphometric and quantitative techniques of the Metamorph program, allowing investigators to count cells, measure processes, and perform a variety of other image analysis techniques that will greatly enhance their research efforts.
The networking capabilities of the computers within the Core allow exporting of images to the Multimedia Core for large format professional printing and for multimedia presentations. The microscopic capabilities of the Imaging Core are well suited to analyze materials generated within the Cellular Neuroscience Core of the MRRC and are used for karyotyping embryonic stem cells of the Gene Manipulation Core.
| Categories and Institutions: |
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| Personnel/Contact Information: |
Director: Scott Pomeroy
Email
Member: Matthew Salanga
Role: System Manager
Email
| Facilities and Equipment: |
Location of Core: Children's Hospital 320 Longwood Avenue Suite 375 Boston, MA 02115
Major Equipment:
- Digital Microscopy System
- Noran Oz Laser Scanning Confocal Microscope
- Silicon Graphics Indy 180 MHZ Workstation
- Three-Dimensional Reconstruction System
| Services: |
- Multilabel fluorescence imaging
- Quantitative calcium imaging
- Three-dimensional visualization and quantitative analysis
- Time-lapse imaging
- Time-lapse imaging and morphometry using light microscopy
- Ultraviolet imaging and uncaging
| Getting Started: |
Telephone: (617) 355-8553
| Departmental Web Link: |
http://core.iddrc.org/imaging/