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DFCI Flow Cytometry Core Facility

Director: Lazo-Kallanian, Suzan

Location: Flow Cytometry Core Facility, Jimmy Fund Building 415, Dana-Farber Cancer Institute, 44 Binney St., Boston, MA 02115

Core Summary:

The Flow Cytometry Core Facility provides sterile sorting and analysis of samples in support of research at DFCI. The core has 2 high-speed cell sorters, the BD FACSAria II SORP and BD FACSAria IIu with up to 12-color simultaneous detection utilizing multi-laser platforms. Sample populations can be sterile sorted into a variety of tubes or plates. The BD FACSAria II SORP and BD FACSAria IIu are both equipped with the Automated Cell Deposition Unit (ACDU) for sterile single cell sorting into 96, 384 micro titer plates.

The core presently has 2 analyzers, the BD LSRFortessa SORP and BD LSRII with up to 12-color simultaneous detection utilizing multi-laser platforms. The BD LSR II is also equipped with an HTS (high throughput sampler) for analyzing samples directly from 96 well micro titer plates as well as being able to run 5ml tubes.

The Facility staff is available to assist researchers with their sorting and analysis needs. Researchers who would like to utilize the analyzers are trained so that they can analyze their experiments independently. The staff will assist with data acquisition and analysis as well as interpretation. They will also assist in experiment design and implementation as well as troubleshooting. Training sessions are available for researchers who would like to run their sorting experiments independently.

Institutions:
  • Dana-Farber Cancer Institute
Personnel/Contact Information:

Director: Lazo-Kallanian, Suzan
Phone: (617) 632-3179
Email

PI: Daley, John
Role: Senior Director
Phone: (617) 632-3139
Email

Member: General Inquiries
Phone: (617) 632-3179
Email

Facilities and Equipment:

Location of Core: Flow Cytometry Core Facility, Jimmy Fund Building 415, Dana-Farber Cancer Institute, 44 Binney St., Boston, MA 02115

Major Equipment:

  • 2 BD FACSCanto II HTS Analyzers (8 Parameter, Mayer Building 584)
  • 2 BD LSRFortessa Analyzers (18 Parameter, Mayer Building 584)
  • BD FACSAria II SORP Cell Sorter (17 parameter, Mayer Building 584)
  • BD FACSAria II SORP Cell Sorter UV (17 parameter, Jimmy Fund Building 415)
  • BD FACSAria II SORP Cell Sorter UV (18 parameter, Mayer Building 584)
  • BD FACSAria IIu Cell Sorter (9 parameter, Jimmy Fund Building 415)
  • BD FACSCanto II Carousel Analyzer (8 Parameter, Mayer Building 584)
  • BD FACSDiva (Jimmy Fund Building 415 and Mayer Building 584)
  • BD LSRFortessa cell analyzer (10 parameter, Jimmy Fund Building 415)
  • BD LSR II cell analyzer (12 parameter, Jimmy Fund Building 415)
  • FlowJo (Jimmy Fund Building 415)
  • Modfit LT (Jimmy Fund Building 415)
Services:
  • Cell Analysis

    * BD LSRFortessa cell analyzer
    - Detection of up to 10 fluorescent parameters
    - 4 laser system (488nm, 640nm, 407nm, 561nm)
    * BD LSR II cell analyzer
    - Detection of up to 12 fluorescent parameters
    - 3 laser system (488nm, 640nm, 407nm)

  • Cell Sorting

    * BD FACSAria IIu cell sorter
    - Detection of up to 9 fluorescent parameters
    - 3 laser system (488nm, 640nm, 407nm)

    * BD FACSAria II SORP
    - Detection of up to 17 fluorescent parameters
    - 6 laser system (488nm, 640nm, 407nm, 455nm, 561nm, 355nm)

  • Data Analysis

    * BD FACSDiva
    * FlowJo analysis software
    * ModFit LT analysis software


Departmental Web Link(s):

Last updated: 2015-02-03T16:44:52.140-06:00