A searchable database of core laboratory facilities. Core Facilities

Genotyping and Genetics for Population Sciences Core (DF/HCC)

Director: De Vivo, Immaculata, M.P.H., Ph.D.

Location: Harvard T.H. Chan School of Public Health, 665 Huntington Ave., Bldg 2. Room 255, Boston MA 02115

Core Summary:

The mission of the Genotyping and Genetics for Population Sciences Core is to provide services to investigators conducting molecular analyses of somatic DNA collected as part of a wide range of investigations. This Core provides high-throughput assays of specific gene mutations and polymorphisms (SNPs) in the many situations where previously defined specific nucleotide alterations are of interest.

Institutions:
  • Dana-Farber/Harvard Cancer Center
Personnel/Contact Information:

Director: De Vivo, Immaculata, M.P.H., Ph.D.
Phone: (617) 525-2094
Email

Member: General Inquiries
Email

Facilities and Equipment:

Location of Core: Harvard T.H. Chan School of Public Health, 665 Huntington Ave., Bldg 2. Room 255, Boston MA 02115

Major Equipment:

  • ABI PRISM 7900HT sequence detection system
  • Ion Torrent PGM
  • Life Technologies Quant 6 Real-Time PCR Machine
  • Life Technologies Quant Studio 12-K Open Array Scanner
  • OpenArray
  • Perkin Elmer Janus liquid Handler
  • Qiagen QIAcube
  • Qiagen QIAgility
Services:
  • Ion Torrent Targeted Sequencing

    The capabilities of this service include:
    Custom Amplicon Sequencing of DNA and RNA Panels
    Ready-to-use Ion AmpliSeq Panels:

    Cancer Hotspot Panel – 50 genes, > 2,800 Cosmic Mutations, 207 Amplicons
    Comprehensive Cancer Panel – 409 genes, 16,000 Amplicons
    Inherited Disease Panel – 700 disease, 328 genes, 10,000 Amplicons

    The system requires only 10ng of input DNA and DNA from FFPE samples can also be used. Additionally, only one or two samples can be run at a time.

  • SNP Analysis using OpenArray Genotyping

    The Taqman OpenArray platform allows users to run 16, 32 or 64 SNPs in parallel using Taqman reagents.

    The per genotype cost varies with the number of samples and the number of SNPs. Please contact Hardeep Ranu for exact pricing information.

  • SNP Analysis using Taqman

    SNP Analysis using ABI Prism Taqman Allelic Discrimination Assay
    Single SNP allelic discrimination is carried out using the ABI 7900, which allows single-plex SNP interrogation over a large volume of samples.

  • Telomere Length Analysis

    Relative telomere length

  • Whole genome amplification service

    DNA and RNA extraction from blood, saliva, tissue, and FFPE plating, and whole genome amplification.


Departmental Web Link(s):

Last updated: 2017-05-09T14:01:30.008-05:00