A searchable database of core laboratory facilities. Core Facilities

Molecular Biology Core Facilities (DFCI)

Director: Morrison, Paul T.

Location: Dana-Farber Cancer Institute, Molecular Biology Core Facilities, 44 Binney Street, Boston, MA 02115

Core Summary:

The Molecular Biology Core Facilities (MBCF) was created in 1986 to allow investigators at the Dana-Farber Cancer Institute access to cutting edge molecular biology tools which would be tested and developed in a shared setting. Collaborations can be set up with anyone in the world. Although these services are primarily focused on cancer and AIDS research, there is a broad spectrum of research that uses these resources.

Institutions:
  • Dana-Farber Cancer Institute
Personnel/Contact Information:

Director: Morrison, Paul T.
Phone: (617) 632-3082
Email

Facilities and Equipment:

Location of Core: Dana-Farber Cancer Institute, Molecular Biology Core Facilities, 44 Binney Street, Boston, MA 02115

Major Equipment:

  • (2) Illumina NextSeq 500 DNA Sequencers
  • (3) Illumina MiSeq DNA Sequencer
  • 4800 MALDI-TOF/TOF mass spectrometer
  • ABI 3730xl DNA analyzer
  • ABI 433A peptide synthesizer
  • Agilent TapeStation 2200
  • Agilent Technologies 1200 Series amino acid analyzer
  • Biacore 3000 analyzer
  • IntegenX Apollo Prep Station
  • Liquid Chromatography - Mass Spectrometer Workstation
  • nanoACQUITY HPLC
  • PE/Applied Biosystems 9700 GeneAmp thermocycler
  • Perseptive Biosystems Voyager DE STR mass analyzer
  • Protein Sequencer
  • Sage Sciences Pippin Prep
  • Tempo LC MALDI
  • ThermoFinnigan LTQ mass spectrometer
Services:
  • Amino acid analysis service

  • Automated DNA sequencing service

  • BIAcore ligand assays service

    Service includes ligand assays, protein-protein interaction, and access to a user operated BIAcore 3000 instrument.

  • Genotyping service

    Service includes polymerase chain reaction.

  • Mass spectrometry service

    Service includes: in gel protein digests and analysis; liquid chromatography; elucidation of post translational modifications; analysis of your HPLC or FPLC fractions, 100 at a time; and access to a current database digested and analyzed with fragment data. Researchers are encouraged to learn how to use the instruments and analyze the data.

  • Next Generation DNA Sequencing using three Illumina MiSeq DNA Sequencers

  • Peptide synthesis service

    Service includes: amino acid analysis, liquid chromatography, analytical HPLC, and preparative HPLC.

  • Protein sequencing service

    Service includes: Westerns, liquid chromatography, protein digests and HPLC peptide mapping, and current database searches.


Departmental Web Link(s):

Last updated: 2015-03-04T09:30:33.474-06:00