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Human Microbe Identification Microarray Core (Forsyth)

Director: Paster, Bruce, Ph.D.

Location: The Forsyth Institute, 245 First Street, Cambridge, MA 02142

Core Summary:

The Microbial Identification Microarray Core (MIM) at The Forsyth Institute is a one-of-a-kind core service that enables the rapid determination of bacterial profiles of human clinical samples. The first MIM offering focuses on the detection of bacterial profiles from clinical samples from the oral cavity. Drs. Bruce Paster and Floyd Dewhirst, have used molecular analyses based on 16S rRNA sequencing to identify about 600 oral bacterial species, of which over half have not yet been cultivated. Using this information, they have developed the Human Oral Microbe Identification Microarray, or HOMIM, which allows the simultaneous detection of about 300 of the most prevalent oral bacterial species, including those that cannot yet be grown in vitro.

Microarrays targeting bacterial species of the human and mouse intestines are presently under development. In addition, exploratory and pilot studies to identify bacteria within any human clinical sample by 16S rRNA cloning and sequencing are available.

This service is available to researchers from all academic institutions and to industry. Researchers submit DNA isolated from clinical samples and receive an online comprehensive data analysis and easy-to-interpret readout. Depending upon the number of samples to be analyzed and position in the queue, results can usually be obtained within days. Note that results are presently for research purposes only.

Institutions:
  • Forsyth Institute
Personnel/Contact Information:

Director: Paster, Bruce, Ph.D.
Phone: (617) 892-8288
Email

Member: Kokaras, Alexis
Phone: (617) 892-8567
Email

Member: General Inquiries
Phone: (617) 892-8288

Services:
  • Comparison of bacterial associations in health vs. disease by microarray

    Determination of bacterial profiles of human clinical samples and comparison of bacterial associations in health vs. disease.

  • Detection of oral species in extra-oral sites

    Investigating for the presence of oral bacterial species in extra-oral sites such as abscesses, atherosclerotic plaques, sepsis, etc.

  • DNA screening from clinical samples

    Researchers submit DNA isolated from clinical samples and receive an online comprehensive data analysis and easy-to-interpret readout.

  • Human microbial identification microarray service

    "Utility of HOMIM:
    - To determine and compare bacterial associations in oral health and disease, including different types of periodontitis, caries, gingivitis, ventilator-associated pneumonia, endodontic and odontogenic lesions, abscesses, and halitosis
    - To determine the efficacy of therapies, e.g., mouth rinses, antibiotic treatment, scaling and root planing, and laser or periodontal surgery
    - To determine the progression of oral diseases
    - To determine those patients at risk for periodontitis and other oral diseases
    - To determine potential biomarkers for non-oral, systemic diseases, e.g., pancreatic cancer, heart disease, and Crohn's Disease

    HOMIM has been used to rapidly produce preliminary data for grants, to obtain data for student projects or theses, and to analyze precious, archived frozen samples (e.g., saliva).

    At this time, HOMIMs may not be used for diagnostic purposes and are to be used only for research endeavors."

  • Performance of microbial perturbation studies

  • Performance of therapeutic monitoring of oral ecology

    We test for the effects of various therapies on the oral ecology.


Departmental Web Link(s):

Last updated: 2011-06-10T10:40:48.138-05:00