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Bauer Core Laboratory (FAS)

Location: Harvard FAS Center for Systems Biology, Northwest Lab Building, 52 Oxford Street, B227.80, Cambridge MA 02138

Core Summary:

Our goal is to advance research efforts in the life sciences that cannot readily be accomplished in the traditional academic laboratory because of a need for expensive instrumentation or automation, scientific or organizational infrastructure, or multidisciplinary expertise.

To promote cutting-edge research and to foster scientific collaborations, we make our extensive laboratory and computational resources available to scientists at Harvard. Our technical staff provide expertise and hands-on training in protocols and the use of instrumentation for a nominal fee. Researchers can sign up to use the instrumentation through an on-line scheduling system and conduct their experiments independently.

Institutions:
  • Harvard Faculty of Arts and Sciences
Personnel/Contact Information:

Member: Reardon, Claire
Role: Lab Manager
Phone: (617) 384-7913
Email

Member: General Inquiries
Email

Facilities and Equipment:

Location of Core: Harvard FAS Center for Systems Biology, Northwest Lab Building, 52 Oxford Street, B227.80, Cambridge MA 02138

Major Equipment:

  • ABI 3130xl genetic analyzer
  • ABI 3730xl DNA analyzer
  • ABI PRISM 6100 nucleic acid prepstation
  • ABI PRISM 7900HT sequence detection system
  • Affymetrix GeneChip System
  • Agilent 2100 Bioanalyzer
  • Agilent Scanner
  • Agilent TapeStation
  • Alpha Innotech ChemiImager
  • AutoGenPrep 965
  • Axon GenePix 4000B Scanner
  • BD FACSAria Cell Sorter
  • BD Fortessa analyzer
  • BD LSR II analyzer
  • Beckman Coulter Biomek FX laboratory automation workstation
  • Beckman Coulter MoFlo Astrios cell sorter
  • Beckman Coulter MoFlo Legacy Cell Sorter
  • Beckman Coulter MoFlo XDP cell sorter
  • Beckman Coulter Multisizer 3
  • Biacore 3000 analyzer
  • BioRad CFX384 Touch Real Time PCR instrument
  • BioRad CFX96 Touch Real Time PCR instrument
  • Bioscreen-C growth curves analysis system
  • BioTek µFill dispensing device
  • Covaris S220 Ultrasonicator
  • Fisher Scientific 550 (Misonix XL2020) sonicator
  • GE Amersham Typhoon imager
  • GeneMachines (DigiLab) Hydroshear
  • GeneMapper
  • Genomic Solutions OmniGrid 100
  • Illumina HiSeq 2000
  • Illumina HiSeq 2500
  • Illumina MiSeq
  • IntegenX Apollo324 Automated NGS Library Preparation System
  • MJ Opticon 2 thermal cycler
  • MJ Tetrad thermal cycler
  • Molecular Devices FlexStation multi-mode plate reader
  • Molecular Devices SpectraMax Gemini XS Plate Reader
  • Molecular Devices Spectramax i3 multi-mode plate reader
  • Molecular Devices Spectramax L luminescent reader
  • Molecular Devices SpectraMax Plus384 Plate Reader
  • NanoDrop ND-1000 Spectrophotometer
  • Qubit Fluorometer
  • Sequencher
  • Stratagene MX3000P thermal cycler
  • Tecan Evo robotic workstation
  • Tecan Safire2 plate reader
  • Thermo MultiDrop
  • Thermo MultiDrop Micro
Services:
  • Cell analysis service

  • Cell sorting service

  • DNA, RNA and protein sample analysis service

  • Fragment analysis

  • Genotyping service

  • Illumina Sequencing Service

    The Center supports massively parallel high throughput sequencing using two Illumina HiSeq 2000 systems. These sequencers can generate billions of bases of data per run and enable a wide variety of applications including

    * DNA sequencing
    * Gene Expression
    * Small RNA
    * ChIP-Seq

    Researchers can choose read lengths of 50 or 100 bp. Paired end reads make sequence mapping easier. Multiple samples can be indexed (barcoded) and be combined in order to make the most of the system's output.

    Each flow cell contains 8 channels, enough for 7 samples and 1 control. All the channels on a flow cell must be used at the same time, but they may be shared by different groups. If you are interested in sharing a flow cell, please talk to us and we'll try to help match you with someone.

  • Library prep for Illumina sequencing service

    The Center supports automated Illumina library preparation with the Apollo 324 system. The system generates libraries for DNA sequencing, RNA-seq or ChIP-seq starting from as little as 200 ng of DNA or 2ng of total RNA (even smaller starting amounts may be used if necessary). The system uses standard enzymes for library creation and magnetic beads for purification. Researchers may be trained to use the system independently. We are currently piloting full-service library preparation performed on the Apollo system by our core staff. If you are interested in full-service library prep, please email illumina_submission-list@lists.fas.harvard.edu.

  • Microarrays service

    The Bauer Core supports Affymetrix GeneChips® and Agilent Microarrays. In addition, the core is equipped for making custom (“homemade”) nucleic acid or protein microarrays using printing technology. Harvard researchers may design and fabricate their own custom microarrays after participating in hands-on training sessions on the instrumentation.

    The Bauer Core has a dedicated laboratory staff to train scientists on the instruments, to provide technical expertise, and to maintain the equipment. We help researchers throughout the process from designing informative experiments to performing sophisticated data analysis.

  • Nucleic acid shearing service

  • Sanger sequencing service


Departmental Web Link(s):

Last updated: 2015-02-04T13:23:13.891-06:00