A searchable database of core laboratory facilities. Core Facilities

Cellular Imaging Core (BCH)

Location: Children's Hospital, Division of Neuroscience, 300 Longwood Avenue, CLS13035, Boston, MA 02115

Core Summary:

The Imaging Core provides access to standard digital and laser scanning confocal microscopy, multiphoton microscopy, unbiased stereology, and automated confocal microscopy for large scale genome-wide screens. In addition, the Core has substantial capabilities in quantitative and three-dimensional volumetric image reconstruction.

Institutions:
  • Boston Children's Hospital
Personnel/Contact Information:

Member: Innocent, Cvic, Ph.D.
Role: Assistant Director of Microscopy
Phone: (617) 919-2378
Email

PI: Chen, Chinfei, M.D., Ph.D.
Role: Associate Professor of Neurology, Director of the Cellular Imaging Core
Phone: (617) 919-2685
Email

Facilities and Equipment:

Location of Core: Children's Hospital, Division of Neuroscience, 300 Longwood Avenue, CLS13035, Boston, MA 02115

Major Equipment:

  • 100X Nikon Plan Fluor
  • 100X Zeiss plan-APOCHROMAT
  • 10X Nikon Plan Apo
  • 10X Nikon Plan Fluor
  • 10X Nikon Plan Fluor
  • 10X Zeiss plan-NEOFLUAR
  • 1X Nikon Plan UW
  • 20X Nikon Plan Apo
  • 20X Nikon Plan Fluor
  • 25X Zeiss plan-NEOFLUAR
  • 2X Nikon Plan Apo
  • 40X Nikon Plan Apo
  • 40X Nikon Plan Fluor
  • 40X Zeiss plan-NEOFLUAR
  • 40X Zeiss plan-NEOFLUAR
  • 458, 488 and 514 nm: Argon-ion laser, 30 mW
  • 4X Nikon Plan Apo
  • 543 nm: Helium-Neon laser, 1mW
  • 5X Zeiss plan-APOCHROMAT
  • 60X Nikon Plan Apo
  • 633 nm: Helium-Neon laser, 15mW
  • 63X Zeiss plan-APOCHROMAT
  • 720-930 nm: tunable Chameleon IR laser, 1,870 mW max (2-photon excitation)
  • Camera lucida
  • CY5 fluorescence filter cube
  • DAPI fluorescence filter cube
  • Digital camera
  • Fiber optics illuminator
  • FITC fluorescence filter cube
  • Hamamatsu ORCA-R2 CCD camera
  • ImageJ
  • Leica Autostainer Xl automatic staining machine
  • Leica CM 3050S cryostat
  • Leica EM UC7 Ultramicrotome
  • Leica SM 2000R freezing sliding microtome
  • LKB glass knife maker
  • LSM 510
  • MetaMorph
  • MetaMorph Advanced Image Analysis PC Workstation
  • Motorized XY stage and Z-axis focus
  • Neurolucida and Stereo Investigator Imaging Station
  • Nikon 80i microscope (air) microscope
  • Nikon 80i microscope (air) workstation
  • Nikon 80i upright microscope
  • Nikon E800 microscope (oil)
  • Nikon E800 microscope (oil) workstation
  • Nikon SZ654 dissecting microscope
  • Nikon SZ654 dissecting microscope workstation
  • Pelco BioWave Pro 36500
  • Photo-camera
  • Reichert-Jung knife sharpener
  • Reichert-Jung rotary 2030 microtome
  • Reichert-Jung rotary 2050 microtome
  • Remote Confocal Image Processing PC Workstation
  • Rhodamin fluorescence filter cube
  • Spot RT cooled CCD camera
  • TRITC fluorescence filter cube
  • Tx Red fluorescence filter cube
  • UV fluorescence filter cube
  • Zeiss Axioplan 2 microscope
  • Zeiss Axioplan 2 microscope workstation
  • Zeiss Axioskop microscope
  • Zeiss Axioskop microscope workstation
  • Zeiss LSM Image Browser

Departmental Web Link(s):

Last updated: 2017-10-25T08:46:45.928-05:00