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DF/HCC Monoclonal Antibody Core

Director: Greenfield, Edward A.

Location: Dana-Farber Cancer Institute, 21/27 Burlington Ave, 4th Floor, Boston, MA 02215

Core Summary:

The mission of the DF/HCC Monoclonal Antibody Core (MAC) is to produce novel monoclonal antibodies that are directed against antigens of interest to Dana-Farber, the Harvard Cancer Center, and affiliated investigators. These monoclonal antibodies may be useful for basic research, drug discovery and clinical applications including diagnosis, surrogate markers for disease status, response to therapy or drug toxicity.

Given the diverse research needs of investigators at DFCI and the Harvard Community, the MAC should strive to support a wide range of antibody requests. A key component of the DF/HCC MAC is the ability to explore and develop new technologies that facilitate generation of monoclonal antibodies. The DF/HCC MAC should be capable of supporting all basic functions of generating, screening, storing and producing monoclonal antibodies.

Institutions:
  • Dana-Farber/Harvard Cancer Center
  • Dana-Farber Cancer Institute
Personnel/Contact Information:

Director: Greenfield, Edward A.
Phone: (617) 632-2209
Email

Member: Nong, Yuhua
Role: Research Scientist
Phone: (617) 582-9152
Email

PI: DeCaprio, James, M.D.
Role: Faculty Advisor
Phone: (617) 582-8601
Email

Member: Brahmandam, Mohan
Role: Research Scientist
Phone: (617) 582-7143
Email

Member: Bencsics, Craig
Role: Research Scientist
Phone: (617) 582-8035
Email

Member: General Inquiries
Email

Services:
  • Cryopreservation service

    *Individual vials
    *Cell master or working bank service

  • ELISA service

    * Direct ELISA
    * Sandwich ELISA

  • Endotoxin service

    Includes:
    * Endotoxin detection
    * Endotoxin removal

  • Flow cytometry service

    Includes flow cytometry screen and staining.

  • Hybridoma fusion

    Includes:
    * Fusion-mouse
    * Fusion-Rat
    * Fusion-Hamster

  • Hybridoma productivity assay service

    Productivity assay-dot blot

  • Isotyping service

  • Mycoplasma testing

  • Production and purification service

    Flask/Roller bottle production only:
    * Standard medium-100ml
    * Standard medium-500ml
    * Standard medium-1000ml

    * Low Ig Medium-100ml
    * Low Ig Medium-500ml
    * Low Ig Medium-1000ml

    * Serum free Medium-100ml
    * Serum free Medium-500ml
    * Serum free Medium-1000ml

    Supernatant Purification only:
    * Antibody Purification 100ml
    * Antibody Purification 500ml
    * Antibody Purification 1000ml

    Integra Flask Production/Purification:
    * Standard medium-100ml
    * Low Ig Medium-100ml
    * Serum free Medium-100ml

    FiberCell Hollow Fiber Production
    * Standard medium-100ml
    * Low Ig Medium-100ml
    * Serum free Medium-100ml

  • Protein development/cloning

    The Protein Development/Cloning Services include:

    * Mammalian Pilot
    * Bacterial Pilot
    * Baculovirus Pliot
    * Molecular Cloning-1 Fragment
    * Molecular Cloning-2 Fragments

  • RIMMS Rapid 21-day Rodent Immunization Service

    Services include:

    * RIMMS-Hamster, Rat or Mouse Immunization
    * Subtractive pre-immunization
    * cDNA immunization
    * Cell line immunization
    * Additional bleed/titer

  • Standard 68-day Rodent Immunization Service

    Services include:

    * Immunization of mice, hamsters, and rats
    * Subtractive pre-immunization
    * cDNA immunization
    * Cell line immunization
    * Additional bleed/titer

  • Subcloning package service

    Subcloning package (per hybridoma). Includes: Subclone / ELISA screen/ freeze 2 vials/ Isotype.

  • Western blot service


Departmental Web Link(s):

Last updated: 2013-05-09T09:08:04.180-05:00